Summary
Dichloromethylene diphosphonate (Cl2MDP) was given at doses of 4 mg/kg and 10 mg/kg daily for 7 days to adult thyroparathyroidectomized rats fed a low calcium diet. Primary metaphyseal trabeculae in Cl2MDP-treated rats were more numerous and longer than in controls. The light and electron microscopic appearance of osteoblasts, osteocytes and osteoclasts were unaltered by Cl2MDP. Bone alkaline phosphatase was significantly elevated in rats given Cl2MDP but adenosine triphosphatase activity was unchanged. Bone fat-free weight, fat-free minus ash weight, and bone calcium and phosphorus concentration were reduced significantly in rats given 10 mg/kg Cl2MDP compared to controls. Bone magnesium concentration was significantly elevated in rats given 10 mg/kg Cl2MDP. Serum calcium and phosphorus concentration were lower in Cl2MDP-treated rats.
These results suggest that Cl2MDP is capable of altering bone remodeling, enzyme activity and mineral content, without significantly altering bone cell morphology, independent of the effects of parathyroid hormone, calcitonin, and dietary calcium.
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Bachra, B.N., Trautz, O.P., Simon, S.L.: Precipitation of calcium carbonate and phosphate III. The effect of magnesium and fluoride ions on the spontaneous precipitation of calcium carbonates and phosphates. Arch. Oral Biol.10, 731–738 (1965).
Baginski, E.S., Foa, P.P., Zak, B.: Determination of phosphate: Study of labile organic phosphate interference. Clin. Chim. Acta15, 155–158 (1967)
Eaton, R.H., Moss, D.W.: Partial purification and some properties of human bone alkaline phosphatase. Enzymologia35, 31–39 (1968)
Fiske, C.H., Subbarow, Y.: The colorimetric determination of phosphorus. J. Biol. Chem.66, 375–400 (1925)
Fleisch, H., Russell, R.G.G., Francis, M.D.: Diphosphonates inhibit hydroxyapatite dissolution in vitro and bone resorption in tissue culture and in vivo. Science165, 1262–1264 (1969)
Francis, M.D., Briner, W.W., Gray, J.A.: Chemical agents in the control of calcification processes in biological systems. In: Hard Tissue Growth, Repair and Remineralization. Ciba Foundation Symposium11, 52–83 (1973)
Francis, M.D., Russell, R.G.G., Fleisch, H.: Diphosphonates inhibit formation of calcium phosphate crystals in vitro and pathologic calcification in vivo. Science165, 1264–1266 (1969)
Hausamen, T.U., Helger, R., Rick, W., Gross, W.: Optimal conditions for determination of serum alkaline phosphatase by a new kinetic method. Clin. Chim. Acta15, 244–245 (1967)
Lowry, O.H., Rosebrough, N.H., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 260–275 (1951)
Morgan, D.B., Monod, A., Russell, R.G.G., Fleisch, H.: Influence of dichloromethylene diphosphonate and calcitonin on bone resorption, lactate production, and phosphatase and pyrophosphatase content of mouse calvaria treated with parathyroid hormone in vitro. Calcif. Tiss. Res.13, 287–294 (1973)
Reynolds, J.J., Murphy, H., Mühlbauer, R.C., Morgan, D.B., Fleisch, H.: Inhibition by diphosphonates of bone resorption in mice and comparison with grey-lethal osteopetrosis. Calcif. Tiss. Res.12, 59–71 (1973)
Rowe, D.J., Hausmann, E.: The alterations of osteoclast morphology by diphosphonates in bone organ culture. Calcif. Tiss. Res.20, 53–60 (1976)
Russell, R.G.G., Fleisch, H.: Pyrophosphate and diphosphonates in skeletal metabolism. Clin. Orthop.108, 241–263 (1975)
Russell, R.G.G., Kislig, A.M., Casey, P.A., Fleisch, H., Thornton, J., Schenk, R., Williams, D.A.: Effect of diphosphonates and calcitonin on the chemistry and quantitative histology of rat bone. Calcif. Tiss. Res.11, 179–195 (1973)
Schenk, R., Merz, W.A., Mühlbauer, R., Russell, R.G.G., Fleisch, H.: Effect of ethane-1-hydroxy-1,1-diphosphonate (EHDP) and dichloromethylene diphosphonate (Cl2MDP) on the calcification and resorption of cartilage and bone in tibial epiphysis and metaphysis of rats. Calcif. Tiss. Res.11, 196–214 (1973)
Weisbrode, S.E., Capen, C.C., Nagode, L.A.: Fine structural and enzymatic evaluation of bone in thyroparathyroidectomized rats receiving various levels of vitamin D. Lab. Invest.28, 29–37 (1973)
Weisbrode, S.E., Capen, C.C., Pendley, C.B.: Interaction of dichloromethylene diphosphonate and vitamin D on bone in thyroparathyroidectomized rats. Amer. J. Path.89, 137–146 (1977)
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Weisbrode, S.E., Capen, C.C. & Pendley, C.B. Effect of dichloromethylene diphosphonate on morphology, enzyme activity, and ash content of bones of thyroparathyroidectomized rats. Calc. Tis Res. 25, 119–126 (1978). https://doi.org/10.1007/BF02010760
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DOI: https://doi.org/10.1007/BF02010760