Microbial Ecology

, Volume 3, Issue 4, pp 289–303 | Cite as

Isolation and characterization of a high molecular weight antibiotic produced by a marine bacterium

  • M. Ballester
  • J. M. Ballester
  • J. P. Belaich
Article

Abstract

Many marine bacteria demonstrate antibiotic activity against organisms of terrestrial origin. Low molecular weight antibiotics have been extracted and, in some cases, purified, but few attempts have been made to isolate high molecular weight antibiotics produced by marine bacteria. In the study reported here, a high molecular weight antibiotic was extracted from whole cells ofAlteromonas strain P18 (NCMB 1890) grown on 2216E medium. Purification included ammonium sulfate precipitation, ultracentrifugation, chromatography on DEAE cellulose, and gel filtration on Ultrogel. A rapid method for measuring specific activity of the antibiotic was developed.

Keywords

Precipitation Cellulose Filtration High Molecular Weight Nature Conservation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Andersen, R.J., Wolfe, M.S., and Faulkner, D.J. 1974. Autotoxic antibiotic production by a marineChromobacterium.Mar. Biol. 27: 281–285.Google Scholar
  2. 2.
    Berland, B. and Maestrini, S. 1969. Study of bacteria associated with marine algae in culture. II. Action of antibiotic substances.Mar. Biol. 3: 334–335.Google Scholar
  3. 3.
    Berland, B.R., Bonin, D.J., and Maestrini, S.Y. 1972. Are some bacteria toxic for marine algae?Mar. Biol. 12: 189–192.Google Scholar
  4. 4.
    Berland, B.R., Bonin, D.J., and Maestrini, S.Y. 1972. Etudes des relations algues-bactéries du milieu marin. Possibilités d'inhibition des algues par les bactéries.Téthys. 4: 339–348.Google Scholar
  5. 5.
    Burkholder, P.R., Pfister, R.M., and Leitz, F.M., 1966. Production of a pyrrole antibiotic by a marine bacterium.Appl. Microbiol. 14: 649–653.PubMedGoogle Scholar
  6. 6.
    Davis, B.J. 1964. Disc eletrophoresis. II. Method and application to human serum proteins.Ann. N.Y. Acad. Sci. 121.Google Scholar
  7. 7.
    De Giaxa, A. 1889. Uber das verhalten einiger pathogener Mikroorganismen im Meerwasser.Z. Hyg. Infecktionkrank. 6: 162–225.Google Scholar
  8. 8.
    Dogget, R.G. 1968. New anti-Pseudomonas agent isolated from a marineVibrio.J. Bacteriol. 95: 1972–1973.PubMedGoogle Scholar
  9. 9.
    Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., and Smith, F. 1956. Colorimetric method for determination of sugars and related substances.Anal. Chem. 28: 350–356.Google Scholar
  10. 10.
    Gauthier, M. 1969. Substances antibactériennes produites par les bactéries marines. 1ère Partie: étude systématique de l'activité antagoniste de souches bactériennes marines vis-à-vis de germes telluriques aérobies.RIOM 15/16: 41–59.Google Scholar
  11. 11.
    Gauthier, M. 1970. Substances antibactériennes produites par les bactéries marines. 2e Partie: lipopolysaccharides antibiotiques produits par certains germes marins appartenant aux genresPseudomonas etChromobacterium.RIOM 17: 23–45.Google Scholar
  12. 12.
    Gauthier, M. 1970. Propriétés antibactériennes des microorganismes marins. Doctorate thesis. Nice University.Google Scholar
  13. 13.
    Gauthier, M.J., Shewan, J.M., Gibson, D.M., and Lee, J. V. 1975. Taxonomic position and seasonal variations in marine neritic environment of some Gram negative antibioticproducing bacteria.J. Gen. Microbiol. 87: 211–218.PubMedGoogle Scholar
  14. 14.
    Gauthier, M.J. 1976. Modification of bacterial respiration by a macromolecular polyanionic antibiotic produced by a marineAltermonas.Antimicrobial Agents and Chemotherapy 9: 361–366.PubMedGoogle Scholar
  15. 15.
    Grein, A. and Meyers, S.P. 1958. Growth characteristics and antibiotic production ofActinomycetes isolated from littoral sediments and materials suspended in sea water.J. Bacterol. 76: 457–463.Google Scholar
  16. 16.
    Jetten, A.M., Vogels, G.D., and de Windt, F. 1972. Production and purification of aStaphylococcus epidermidis bacteriocin.J. Bacteriol. 112: 235–242.PubMedGoogle Scholar
  17. 17.
    Lowry, O.H., Rosebrough, N.I., Farr, A.L., and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193: 265–275.PubMedGoogle Scholar
  18. 18.
    Mayr-Harting, A., Hedges, A.J., and Berkeley, R.C.W. 1972. Methods for studying bacteriocins. Methods in Microbiology. Edited by Norris-Ribbons. Vol. 7A. Academic Press, New York.Google Scholar
  19. 19.
    Mitchell, R., Yankofsky, S., and Jannasch, H.W. 1967. Lysis ofE. coli by marine microorganisms.Nature (London) 215: 891–893.Google Scholar
  20. 20.
    Nicati, W. and Reitsch, W. 1885. Expériences sur la vitalité du bacille virgule cholérique.Revue Hyg. Police Sanit. 7: 353–378.Google Scholar
  21. 21.
    Ornstein, L. 1964. Disc electrophoresis. Part I.Ann N.Y. Acad. Sci. 121: 321.PubMedGoogle Scholar
  22. 22.
    Rosenfeld, D.W. and Zobell, C.E. 1947. Antibiotic production by marine microorganisms.J. Bacteriol. 54: 393–398.Google Scholar
  23. 23.
    Vaccaro, R.F., Briggs, M.P., Carey, C.L., and Ketchum, B.H. 1950. Viability ofE. coli in sea water.Amer. J. Public. Health 40: 1257–1266.PubMedGoogle Scholar
  24. 24.
    Wood, E.J.F. 1953. Heterotrophic bacteria in marine environments of Eastern Australia.Austr. J. Mar. Freshwater Res. 4: 160–200.Google Scholar
  25. 25.
    Zacharius, R.M., Zell, T.E., Morrison, J.H., and Woodlock, J.J. 1969. Glycoprotein staining following electrophoresis on acrylamide gels.Anal. Biochem. 30: 148–152.PubMedGoogle Scholar
  26. 26.
    Zobell, C.E. 1936. Bactericidal action of sea water.Proc. Soc. Exp. Biol. Med. 34: 113–116.Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1977

Authors and Affiliations

  • M. Ballester
    • 1
  • J. M. Ballester
    • 1
  • J. P. Belaich
    • 1
  1. 1.Laboratoire de Chimie BactérienneC.N.R.S.Marseille Cedex 2France

Personalised recommendations