Microbial Ecology

, Volume 6, Issue 2, pp 125–140 | Cite as

Characterization, distribution, and significance ofMetallogenium in Lake Washington

  • Eileen Gregory
  • R. S. Perry
  • J. T. Staley
Article

Abstract

During summer stratification,Metallogenium personatun was found exclusively in the hypolimnion of Lake Washington where the oxygen tension was below 8 ppm. Numbers of the organism decreased in the lake immediately following turnover in October. Significant concentrations ofMetallogenium microcolonies did not recur until spring, after the lake had stratified. During stratification the distribution of particulate manganese closely followed the distribution ofMetallogenium. EDAX analysis, confirmed by electron microprobe analyses of the encrustation, showed that the primary component was manganese. Iron and some trace elements were also precipitated on the organism but to a lesser degree. In addition, phosphate, the primary substance limiting phytoplankton growth in Lake Washington, was found in the encrustation, indicatingMetallogenium maybe important in limiting algal blooms in the lake. Attempts to growMetallogenium in the laboratory were unsuccessful. This inability, combined with the negative results of thin-sectioning and acridine orange staining ofMetallogenium microcolonies, suggests that the microcolonial structures seen in Lake Washington are not a living form of an organism.

Keywords

Manganese Phytoplankton Stratification Oxygen Tension Acridine 

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References

  1. 1.
    Bromfield, S. M.: Bacterial oxidation of manganous ions as affected by organic substrate concentration and composition. Soil Biol. Biochem.6, 383–394 (1974)CrossRefGoogle Scholar
  2. 2.
    Crecelius, E. A.: The geochemical cycle of arsenic in Lake Washington and its relationship to other elements. Limnol. Oceanogr.20, 441–451 (1975).Google Scholar
  3. 3.
    Dondero, N. C.: TheSphaerotilus-Leptothrix group. Annu. Rev. Microbiol.29, 407–428 (1975)CrossRefPubMedGoogle Scholar
  4. 4.
    Drabkova, V. G., and E. A. Stravinskaya: Role of bacteria in the dynamics of iron in Lake Krasnoe. Mikrobiologiya38, 364–371 (1969) (in Russian)Google Scholar
  5. 5.
    Dubinina, G. A.: Inclusion ofMetallogenium among the Mycoplasmatales. Doklady Akademii NaukSSSR. (Doklady Biological Sciences English Translation)184, 87–90 (1969)Google Scholar
  6. 6.
    Dubinina, G. A.: X. Ecology of freshwater organisms. 1. Aquatic bacteria. The significance of microbiological processes in lakustrine manganese ore formation. Verb. Int. Verein. Limnol.18, 1261–1276 (1973)Google Scholar
  7. 7.
    Edmondson, W. T.: Nutrients and phytoplankton in Lake Washington. In G. E. Likens (ed.): Nutrients and Europhication. Am. Soc. Limnol. Oceanogr. Spec. Symp. I (1972)Google Scholar
  8. 8.
    Guseva, K. A.: Two planktonic microorganisms participating in the iron cycle. Trans. Biol. Station Borok.2, 24–31 (1956) (in Russian)Google Scholar
  9. 9.
    Hobbie, J. E., R. J. Daley, and S. Jasper: Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol.33, 1225–1228 (1977)PubMedGoogle Scholar
  10. 10.
    Hutchinson, G. E.: A Treatise on Limnology, Vol. I. John Wiley and Sons, London (1957)Google Scholar
  11. 11.
    Jones, J. G.: Studies on freshwater bacteria: effect of medium composition and method on estimates of bacterial population. J. Appl. Bacteriol.33, 679–686 (1970)PubMedGoogle Scholar
  12. 12.
    Klaveness, D.: Morphology, distribution and significance of the manganese-accumulating microorganismMetallogenium in lakes. Hydrobiologia56, 25–33 (1977)CrossRefGoogle Scholar
  13. 13.
    Kossaya, T. A.: Composition of manganese oxides in cultures ofMetallogenium andLeptothrix. Mikrobiologiya36, 1024–1029 (1967) (in Russian)Google Scholar
  14. 14.
    Lebedeva, M. N., and G. V. Shumakova: Reliability of data obtained by the method of direct bacterial count on filters. Mikrobiologiya38, 351–357 (1969) (in Russian)Google Scholar
  15. 15.
    Lund, J. W., C. Kipling, and E. D. LeCren: The inverted microscope method of estimating algal numbers and the statistical basis of estimations by counting. Hydrobiologia11, 143–170 (1958)CrossRefGoogle Scholar
  16. 16.
    Merek, E. L.: Imaging and life detection. BioScience23, 364–371 (1973)Google Scholar
  17. 17.
    Perfil'ev, B. V., and D. R. Gabe: Capillary Methods of Investigating Microorganisms. University of Toronto Press, Toronto (1961) (English translation, 1967)Google Scholar
  18. 18.
    Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–212 (1963)CrossRefPubMedGoogle Scholar
  19. 19.
    Shapiro, J., W. T. Edmondson, and D. E. Allison: Changes in the chemical composition of sediments of Lake Washington, 1958–1970. Limnol. Oceanogr.16, 437–452 (1971)Google Scholar
  20. 20.
    Sokolova, G. A.: Seasonal changes in the species composition and numbers of iron bacteria and iron cycle in Lake Glubokoye. Trudy Vsesojoznovo Gidrobiol. Oblschestva11, 5–11 (1961) (in Russian)Google Scholar
  21. 21.
    Sokolova-Dubinina, G. A., and Z. P. Deryugina: Process of iron-manganese concretion formation in Lake Punnus-Yarvi. Mikrobiologiya36, 1066–1076 (1967) (in Russian)Google Scholar
  22. 22.
    Staley, J. T.: Incidence of prosthecate bacteria in a polluted stream. Appl. Microbiol.22, 496–502 (1971)PubMedGoogle Scholar
  23. 23.
    Taras, M. J., A. E. Greenberg, R. D. Hoak, and M. C. Rand: Standard Methods for the Examination of Waterand Wastewater, 13th Ed. American Public Health Assoc., Washington, D. C. (1971)Google Scholar
  24. 24.
    Van Veen, W. L.: Factors affecting the oxidation of manganese bySphaerotilus discophorus. Antonie van Leeuwenhoek J. Microbiol. Serol.38, 623–626 (1972)Google Scholar
  25. 25.
    Walsh, F., and R. Mitchell: An acid-tolerant iron-oxidizingMetallogenium. J. Gen. Microbiol.72, 369–376 (1972)Google Scholar
  26. 26.
    Zavarzin, G. A.: Symbiotic culture of a new microorganism oxidizing manganese. Mikrobiologiya30, 393–395 (1961) (in Russian)Google Scholar
  27. 27.
    Zavarzin, G. A.: Structure ofMetallogenium. Mikrobiologiya32, 1020–1023 (1963) (in Russian)Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1980

Authors and Affiliations

  • Eileen Gregory
    • 1
  • R. S. Perry
    • 1
  • J. T. Staley
    • 1
  1. 1.Department of Microbiology and Immunology, and Department of Geological SciencesUniversity of WashingtonSeattle

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