Microbial Ecology

, Volume 7, Issue 3, pp 275–280 | Cite as

Ecological study of twoNitrobacter serotypes coexisting in the same soil

  • A. Josserand
  • G. Gay
  • G. Faurie


The problem of competition betweenNitrobacter strains was posed by the fact that two serotypes (N.w and L) coexisted in a soil, as shown by immunofluorescence. The L serotype had a lower growth rate in pure culture than serotype N.w, as well as a slower nitrifying activity when inoculated to a sterilized soil percolated with NO2 solution. When both serotypes were inoculated together, only N.w was observed by immunofluorescence at the end of the percolation; when in the control soil the two serotypes were identified, strain L always adhered to particles. Maintenance of the two serotypes in the soil could be explained as their exploitation of distinct ecological niches. Thus the relative in situ importance of the two strains is governed by environmental conditions.


Growth Rate Nature Conservation Pure Culture Ecological Study Ecological Niche 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Belser, W. B.: Population ecology of nitrifying bacteria. Annu. Rev. Microbiol.33, 309–333 (1979)PubMedGoogle Scholar
  2. 2.
    Belser, L. W., and E. L. Schmidt: Serological diversity within a terrestrial ammonia-oxidizing population. Appl. Environ. Microbiol.36, 589–593 (1978)Google Scholar
  3. 3.
    Bohlool, B. B., and E. L. Schmidt: Fluorescent antibody technique for determination of growth rates of bacteria in soil. Bull. Ecol. Res. Commun. (Stockh.)17, 67–76 (1973)Google Scholar
  4. 4.
    Buchanan, R. E., and N. E. Gibbons: Bergey's Manual of Determinative Bacteriology, 8 th Ed. Williams & Wilkins Co., Baltimore (1974)Google Scholar
  5. 5.
    Faurie, G.: Devenir de l'ammonium en sol calcaire: nitrification et volatilisation de l'ammoniac. Thèse d'état, Université de Lyon, France (1980)Google Scholar
  6. 6.
    Faurie, G., and R. Bardin: Modification apportée à un appareil de percolation. Bull. Int. Inf. Biol. Sol.14, 38–40 (1971)Google Scholar
  7. 7.
    Fliermans, C. B., B. B. Bohlool, and E. L. Schmidt: Autoecological study of the chemoautotrophNitrobacter by immunofluorescence. Appl. Microbiol.27, 24–129 (1974)Google Scholar
  8. 8.
    Hobbie, J. E., R. J. Daley, and S. Jasper: Use of Nuclepore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol.33, 1225–1228 (1977)PubMedGoogle Scholar
  9. 9.
    Josserand, A., and J. C. Cleyet-Marel: Isolation from soils ofNitrobacter and evidence for novel serotypes using immunofluorescence. Microb. Ecol.5, 197–205 (1979)Google Scholar
  10. 10.
    Nishio, M., and C. Furusaka: The distribution of nitrifying bacteria in soil aggregates. Soil Sci. Plant Nutr.16, 24–29 (1970)Google Scholar
  11. 11.
    Rennie, R. J., and E. L. Schmidt: Autoecological and kinetic analysis of competition between strains ofNitrobacter in soils. Ecol. Bull. (Stockh.)25, 431–441 (1977)Google Scholar
  12. 12.
    Rider, B. F., and M. G. Mellon: Colorimetric determination of nitrite. Ind. Eng. Chem. Anal. Ed.18, 96–99 (1946)Google Scholar
  13. 13.
    Schmidt, E. L., R. O. Bankole, and B. B. Bohlool: Fluorescent antibody approach to study ofRhizobia in soil. J. Bacteriol.95, 1987–1992 (1968)PubMedGoogle Scholar
  14. 14.
    Schmidt, E. L., J. A. E. Molina, and C. Chiang: Isolation of chemoautotrophic nitrifiers from moroccan soils. Bull. Ecol. Res. Commun. (Stockh.)17, 166–167 (1973)Google Scholar
  15. 15.
    Van Veen, J. A.: The behavior of nitrogen in soil: a computer simulation model. Ph. D. Thesis, Wageningen, The Netherlands (1977)Google Scholar

Copyright information

© Springer-Verlag New York Inc 1981

Authors and Affiliations

  • A. Josserand
    • 1
  • G. Gay
    • 1
  • G. Faurie
    • 1
  1. 1.Laboratoire de Biologie des Sols, E.R.A. 848Université Lyon IVilleurbanneFrance

Personalised recommendations