Abstract
Injection into chick embryos incubated for nine days, of β-aminopropionitrile (BAPN) at doses above 0.312 mg/embryo prevented the formation of the cartilage normally present on the membrane bones from the eleventh day of incubation onwards. The histogenesis of this inhibition is described. BAPN increased the proportion of soluble collagen without altering total collagen synthesis and decreased the synthesis of hexosamine-containing acid mucopolysaccharides within the membrane bones. The relationship between cross-linking of collagen, collagen solubility, synthesis of acid mucopolysaccharides and mechanical stimulation (the extrinsic factor initiating chondrogenesis in this system) in the formation of cartilage from bipotential germinal cells on the membrane bones is discussed.
Résumé
L'injection d'embryons de poulet, incubés pendant neuf jours, avec du β-aminopropionitrile (BAPN), à des doses supérieures à 0,312 mg par embryon, empêche la formation de cartilage, normalement présent au niveau des os de membrane à partir du llème jour de l'inculation. L'hitsogenese de cette inhibition est décrite. BAPN augmente la propertion de collagène et diminue la synthèse des mucopolysaccharides acides, contenant de l'hesoxamine, au niveau des os de membrane. Le rapport entre les chainons tranversaux du collagène, sa solubilité, la synthèse des mucopolysaccharides acides et la stimulation mécanique (le facteur extrinsèque déclenchant la chondrogenèse dans le système) est discuté sous l'angle de la formation cartilagineuse sur les os de membrane, à partir de cellules souches.
Zusammenfassung
Wird β-Aminopropionitrol (BAPN) in Dosen von über 0,312 mg/Embryo in 9 Tage inkubierte Hühnerembryonen injiziert, so wird die Bildung des normalerweise vom 11. Inkubationstag an auf dem Deckknochen vorliegenden Knorpels verhindert. Die Histogenese dieser Hemmung wird beschrieben. BAPN erhöhte den Anteil des löslichen Kollagens, ohne die Synthese des Totalkollagens zu verändern; es verminderte die Synthese der hexosaminhaltigen sauren Mucopolysaccharide innerhalb der Deckknochen. Die Diskussion betrifft die Beziehung zwischen der Quervernetzung des Kollagens, der Kollagenlöslichkeit, der Synthese von sauren Mucopolysacchariden und der mechanischen Stimulation (wobei der Extrinsicfaktor die Chondrogenese in diesem System auslöst) bei der Knorpelbildung aus den bipotentialen Keimzellen der Deckknochen.
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References
Berntsen, E.: Epiphyseal growth zones in acute lathyrism. Determinations of water, hydroxyproline and mucopolysaccharides. Proc. Soc. exp. Biol. (N.Y.)123, 588–592 (1966).
Burdi, A. R., Flecker, K.: Differential staining of cartilage and bone in the intact chick embryonic skeletonin vitro. Stain Technol.43, 47–48 (1968).
Chacko, S., Abbott, J., Holtzer, S., Holtzer, H.: The loss of phenotypic traits by differentiated cells. VI. Behaviour of the progeny of a single chondrocyte. J. exp. Med.130, 417–442 (1969).
Elson, L. A., Morgan, W. T.: A colorimetric method for the determination of glucosamine and chondrosamine. Biochem. J.27, 1824–1828 (1933).
Evans, H. E.: Clearing and staining small vertebratesin toto for demonstration of ossification. Turtox News26, 42–47 (1948).
Golub, L., Stern, B., Glimcher, M., Goldhaber, P.: The inhibition of the maturation of newly synthesized bone collagen by β-aminopropinonitrile in tissue culture. Proc. Soc. exp. Biol. (N.Y.)129, 465–469 (1968).
Hall, B. K.: The formation of adventitious cartilage by membrane bones under the influence of mechanical stimulation appliedin vitro. Life Sci.6, 663–667 (1967).
—: Histochemical aspects of the differentiation of adventitious cartilage on the membrane bones of the embryo chick. Histochem.16, 206–220 (1968a).
—:In vitro studies on the mechanical evocation of adventitious cartilage in the chick. J. exp. Zool.168, 283–306 (1968b).
—: The fate of adventitious and embryonic articular cartilage in the skull of the common fowl,Gallus domesticus (Aves: Phasianidae). Aust. J. Zool.16, 795–805 (1968c).
—: Differentiation of cartilage and bone from common germinal cells. I. The role of acid mucopolysaccharide and collagen. J. exp. Zool.173, 383–394 (1970a).
—: The origin of cartilage and bone from common stem cells. Calcif. Tiss. Res.4 (Suppl.), 147 (1970b).
—: Cellular differentiation in skeletal tissues: a review. Biol. Rev.45, 455–484 (1970c).
Hall, B. K.: Thallium-induced achondroplasia in the embryonic chick. Develop. Biol., in press (1971a).
Hall, B. K.: Skeletal defects in embryonic chicks induced by administration of Beta-aminopropionitrile. Teratol., in press (1971b).
—: Histogenesis and morphogenesis of bone. Clin. Orthop.74, 249–268 (1971c).
Ham, A. W., Harris, W. R.: Histological technique for the study of bone and some notes on the staining of cartilage. In: Handbook of microscopical technique (R. McClung Jones, ed.), p. 269–284. N. Y.: Hoeber Inc. 1950.
Levene, C. I.: The hydrating effect of lathyrogenic compounds on chick-embryo cartilagein vivo. Biochem. J.101, 441–447 (1966).
—, Gross, J.: Alterations in the state of molecular aggregation of collagen induced in chick embryos by β-aminopropinonitrile (lathyrus factor). J. exp. Med.110, 771–790 (1959).
—, Kranzler, J., Franco-Browder, S.: Acid mucopolysaccharides of chick embryo cartilage in osteolathyrism. Biochem. J.101, 435–440 (1966).
Lison, L.: Alcian Blue 8 G with Chlorantine Fast Red 5 B: a technic for selective staining of mucopolysaccharides. Stain Technol.29, 131–138 (1954).
Martin, G. R., Gross, J., Piez, K. A., Lewis, M. S.: On the intramolecular cross-linking of collagen in lathyritic rats. Biochem. biophys. Acta (Amst.)53, 599–601 (1961).
Marzullo, G., Lash, J. W.: Control of phenotypic expression in cultured chondrocytes: Investigations on the mechanism. Develop. Biol.22, 638–654 (1970).
McCallum, H. M., Arbuthnott, J. P.: The inhibition of cartilage matrix production by glutaminoacetonitrilein vitro. Exp. molec. Path.11, 232–246 (1969).
Medoff, J.: Enzymatic events during cartilage differentiation in the chick embryonic limb bud. Develop. Biol.16, 118–143 (1967).
Miller, E. J., Matukas, V. J.: Chick cartilage collagen: a new type of α1 chain not present in bone or skin of the species. Proc. nat. Acad. Sci. (Wash.)64, 1264–1268 (1969).
Murrary, P. D. F., Smiles, M.: Factors in the evocation of adventitious (secondary) cartilage in the chick embryo. Aust. J. Zool.13, 351–381 (1965).
Neuman, R. E., Logan, M. A.: The determinatkon of hydroxyproline. J. biol. Chem.184, 299–306 (1950).
Noback, G. J.: The use of the van Wijhe method for the staining of the cartilaginous skeleton. Anat. Rec.11, 292–294 (1916).
Pantin, C. F. A.: Notes on Microscopical Techniques for Zoologists. C.U.P., 77 pp. (1960).
Prockop, D. J., Pettengill, O., Holtzer, H.: Incorporation of sulfate and the synthesis of collagen by cultures of embryonic chondrocytes. Biochim. biophys. Acta (Amst.)83, 189–196 (1964).
Siegel, R. C., Martin, G. R.: Enzymatic cross-linking of bone collagen. Calcif. Tiss. Res.4, (suppl.), 42 (1970).
Toole, B. P.: Solubility of collagen fibrils formedin vitro in the presence of sulphated acid mucopolysaccharide-protein. Nature (Lond.)222, 872–873 (1969).
Urist, M. R.: A morphogenetic matrix for differentiation of bone tissue. Calcif. Tiss. Res.4 (Suppl.), 98–101 (1970).
—, Sierra, J., de la, Strates, B. S.: The substratum for new bone formation in tendon. Clin. Orthop. Rel. Res.63, 210–221 (1969).
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Hall, B.K. Differentiation of cartilage and bone from common germinal cells. Calc. Tis Res. 8, 276–286 (1971). https://doi.org/10.1007/BF02010147
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DOI: https://doi.org/10.1007/BF02010147