Calcified Tissue Research

, Volume 8, Issue 1, pp 133–141 | Cite as

The experimental induction of parathyroid hyperplasia in rats, and the use of toluidine blue O forin vivo identification of parathyroid tissue

  • Robert H. Whitaker
Original Papers


An experiment was designed to compare the degree of parathyroid hyperplasia induced in rats by subtotal nephrectomy and by oral phosphate administration singly or in combination. The greatest degree of parathyroid hyperplasia was×4.49 and was seen in the rats with both subtotal nephrectomy and oral phosphate administration. Over the 77-day period of the experiment, subtotal nephrectomy alone did not produce a significant increase in the size of the glands, whereas phosphate fed to otherwise normal rats did produce a significant increase.

Toluidine blue was given intravenously before killing the rats and was found to stain the hyperplastic glands in the same way that it stains normal and adenomatous glands. Staining was found to be satisfactory for identification purposes in both the normal and in the hyperplastic glands with 5 mg/kg body weight. A greater depth of staining was given by 10 mg/kg body weight.

Key words

Hyperplasia Histology Parathyroid Induction 


Le degré d'hyperplasie parathyroidïenne a été étudié chez le Rat après néphrectomie subtotale, après administration buccale de phosphate ou par combinaison des deux. Le degré le plus élevé d'hyperplasie parathyroidïenne est de ×4.49 et a été observé chez le rat après néphrectomie sub-totale et administration buccale de phosphate.

Au cours des77 jours d'expérience, la néphrectomie sub-totale ne produit pas une augmentation significative de la taille des glandes, alors que le phosphate, mélangé à l'alimentation de rats normaux, produit une augmentation significative.

Le bleu de toluidine, administré par voie intra-veineuse avant le sacrifice des rats, colore les glandes hyperplasiques de la même façon qu'il colore les glandes normales et adénomateuses. La coloration s'avère intéressante pour l'identification des glandes normales et hyperplasiques à la concentration de 5 mg/kg de poids corporel. Une coloration plus intense est obtenue avec 10 mg/kg de poids corporel.


Es wurde ein Experiment an Ratten durchgeführt, um den Grad der Parathyreoidea-Hyperplasie anhand folgender Punkte zu vergleichen: subtotale Nephrektomie, orale Phosphatverabreichung oder eine Kombination von beiden. Die maximale Parathyreoidea-Hyperplasie war 4,49mal größer als bei den Kontrollen und wurde bei Ratten mit subtotaler Nephrektomie und mit oraler Phosphatverabreichung festgestellt.

Während der 77 Tage des Experimentes erzeugte subtotale Nephrektomie allein keine bedeutende Zunahme der Drüsengröße; Phosphat hingegen, welches im übrigen normalen Ratten verfüttert wurde, bewirkte eine bedeutende Zunahme.

Toluidinblau wurde den Ratten intravenös injiziert, bevor sie getötet wurden; es färbte die hyperplastischen Drüsen ebenso befriedigend wie normale und adenomatöse Drüsen. Die Färbung war mit 5 mg/kg Körpergewicht befriedigend zur Identifizierung der normalen wie der hyperplastischen Drüsen. Eine tiefere Färbung wurde mit 10 mg/kg Körpergewicht erreicht.


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Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • Robert H. Whitaker
    • 1
  1. 1.Department of UrologyThe London HospitalLondon

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