Abstract
Previous investigators have reported a number of methods which will differentially stain peritubular matrix in undecalcified ground sections of human dentin. The methods, however, were all carried out at pH values which were potentially high enough to decalcify the highly acid-soluble peritubular matrix. Microradiograms were prepared of ground sections both before and after they had been exposed to either the staining or pre-staining treatments which reportedly had been successful for staining peritubular matrix. The radiograms revealed that the peritubular matrix was decalcified either at the end of, or long before, the prescribed treatment had been completed. It was concluded that the use of these staining techniques should be re-evaluated by more recent methods.
Résumé
Un certain nombre de méthodes ont été décrites pour colorer de façon différente la matrice péricanaliculaire sur des coupes par usure de dentine humaine. Ces méthodes opèrent toutes à des pH qui sont susceptibles de décalcifier cette matrice. Des microradiographies de coupes par usure ont été réalisées avant et après coloration ou précoloration, ayant permis d'identifier la matrice péricanaliculaire. Les radiographies ont montré que cette matice est décalcifiée avant et à la fin du traitement. De telles techniques de coloration doivent être remplacées par des méthodes plus adaptées.
Zusammenfassung
Verschiedene Forscher haben eine Anzahl von Methoden mitgeteilt, um die peritubuläre Matrix unentkalkter Schliffe von menschlichem Dentin differenziert zu färben. Allerdings wurden sämtliche dieser Methoden bei pH-Werten ausgeführt, die so hoch sind, daß die Möglichkeit besteht, die sehr säurelösliche peritubuläre Matrix zu entkalken. Von Schliffen wurden Mikroradiographien hergestellt, und zwar vor und nach den verschiedenen Färbeprozessen, welche nachgewiesenermaßen erfolgreich zum Färben von peritubulärer Matrix angewandt worden sind. Die Radiographien zeigten, daß die peritubuläre Matrix entweder am Schluß der vorgeschriebenen Behandlung oder sogar schon längst vorher entkalkt war. Es wurde daraus geschlossen, daß diese Färbetechniken durch neuere Methoden ersetzt werden sollten.
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Weber, D.F. Peritubular matrix: A correlated radiographic and staining study. Calc. Tis Res. 8, 17–23 (1971). https://doi.org/10.1007/BF02010119
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DOI: https://doi.org/10.1007/BF02010119