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Failure to detect silent HIV infection by polymerase chain reaction in subjects at risk for heterosexually transmitted HIV type 1 infection

  • F. Luque
  • M. Leal
  • J. A. Pineda
  • Y. Torres
  • I. Aguado
  • M. Olivera
  • J. Hernandez-Quero
  • A. Sanchez-Quijano
  • C. Rey
  • E. Lissen
Article

Abstract

The prevalence of silent HIV-1 infections in subjects at risk of acquiring HIV infection by heterosexual transmission was assessed using a nested polymerase chain reaction (PCR) assay. The two groups of risk subjects consisted of 92 female prostitutes and 43 heterosexual partners of infected individuals. Appropriate positive and negative control persons were included in the study. Serum samples were also tested for antibody to HIV-1 by an enzyme immunoassay (EIA) and positive results confirmed by Western blot. PCR results in the two risk groups and the positive and negative controls were in full agreement with serological results. It is concluded that silent infection with HIV-1 is infrequent in persons at risk for heterosexual transmission.

Keywords

Polymerase Chain Reaction Western Blot Negative Control Serum Sample Risk Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1993

Authors and Affiliations

  • F. Luque
    • 1
  • M. Leal
    • 2
  • J. A. Pineda
    • 2
  • Y. Torres
    • 1
  • I. Aguado
    • 2
  • M. Olivera
    • 1
  • J. Hernandez-Quero
    • 3
  • A. Sanchez-Quijano
    • 2
  • C. Rey
    • 1
  • E. Lissen
    • 2
  1. 1.Department of BiochemistryVirgen del Rocio University HospitalSevilleSpain
  2. 2.Department of Internal MedicineVirgen del Rocio University HospitalSevilleSpain
  3. 3.Infectious Diseases UnitUniversity HospitalGranadaSpain

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