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The Journal of Membrane Biology

, Volume 105, Issue 2, pp 165–175 | Cite as

Stimulation of intestinal Na+/d-glucose cotransport by monoclonal antibodies

  • Konrad Honold
  • Barbara Ludeke
  • Hans Hengartner
  • Giorgio Semenza
Articles

Summary

The small intestinal brush border membrane is endowed with a number of transport systems. Monoclonal antibodies were produced against integral membrane proteins and tested for their ability to bind to such membranes. For this purpose papain-digested, deoxycholate-extracted BBMVs from rabbit small intestine were used to immunize mice. Of the 765 hybridoma supernatants tested, 119 gave a significantly higher extent of binding to the crude antigen preparation as compared with the background. The monoclonal antibodies were also tested for their ability to influence the sodium-dependent uptake of solutes into intact BBMVs. Two monoclonal antibodies clearly showed stimulation of secondary actived-glucose transport, whereas sodium-dependent uptake ofl-alanine andl-proline was not affected. Hydrophobically labeled, i.e. intrinsic, membrane proteins of 175, 78 and 65 kilodaltons could be immunoprecipitated by both monoclonal antibodies, the 78 kDa band corresponding in all likelihood to the Na+/glucose cotransporter.

Key Words

brush border membranes sodium-dependent cotransport monoclonal antibodies immunoprecipitation 

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Copyright information

© Springer-Verlag New York Inc 1988

Authors and Affiliations

  • Konrad Honold
    • 1
  • Barbara Ludeke
    • 1
  • Hans Hengartner
    • 2
  • Giorgio Semenza
    • 1
  1. 1.Laboratory for Biochemistry of the ETHETH-ZentrumZürichSwitzerland
  2. 2.Institute for Pathology, Department of Experimental PathologyUniversity Hospital of ZürichZürichSwitzerland

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