Mode of activation of the metabolic burst in polymorphonuclear leukocytes by calcium oxalate crystals
Microcrystals of calcium oxalate cause an activation of the metabolic burst in polymorphonuclear leukocytes, measured as NBT reduction. Crystal-induced NBT reduction, which was mainly due to superoxide release, was accompanied by enzyme release. Modulation of calcium oxalate-induced activation by several agents resembles the effect of these agents on activation by opsonized zymosan and by phorbol myristate acetate. Both the activation of the metabolic burst and concomitant enzyme release could be counteracted by certain anions, such as oxalate, pyruvate and citrate, indicating that positive charges on the crystals play an important role in crystal-cell interaction. Removal of the negatively charged sialic acid from the cell surface by neuraminidase did not affect the action of the crystals. The mechanism, by which calcium oxalate activates polymorphonuclear leukocytes, is discussed.
KeywordsCalcium Citrate Superoxide Pyruvate Oxalate
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- G. Weissmann, R. B. Zurier, P. J. Spieler and J. H. Goldstein,Mechanisms of lysosomal enzyme release from leukocytes exposed to immune complexes and other particles, J. Exp. Med.134, 1493–1655 (1971).Google Scholar
- M. A. Boogaerts, D. E. Hammerschmidt, C. Roelant, R. L. Verwilghen and H. S. Jacob,Mechanisms of vascular damage in gout an oxalosis: crystal-induced, granulocyte-mediated endothelial injury, Thromb. Haemostas.50, 576–580 (1983).Google Scholar
- N. D. Adams, G. F. Carrera, R. P. Johnson, R. Latorraea and J. Lemann,Calcium oxalate crystal-induced bone disease, Amer. J. Kidney Dis.1, 294–299 (1982).Google Scholar
- J. G. R. Elferink and M. Deierkauf,Enzyme release from polymorphonuclear leukocytes during the interaction with calcium oxalate microcrystals, J. Urol. (1987) in press.Google Scholar
- J. C. Fantone and P. A. Ward,Role of oxygen-derived free radicals and metabolites in leukocytes-dependent inflammatory reaction, Amer. J. Pathol.107, 397–418 (1982).Google Scholar
- J. G. R. Elferink and M. Deierkauf,Inhibition of polymorphonuclear leukocyte functions by chloretracycline, Biochem. Pharmacol.33, 3667–3673.Google Scholar
- Gmelin's Handbuch der Anorganischen Chemie, Nr. 28.Calcium Teil B, Lieferung 3. Verlag Chemie, Weinheim, 1961, p. 998.Google Scholar
- J. G. R. Elferink and J. C. Riemersma,Hydroxyapatite crystal-induced membrane damage in granulocytes and erythrocytes, Agents Actions13, 515–516 (1983).Google Scholar
- A. Deisseroth and A. L. Dounce,Catalase: physical and chemical properties: mechanism of catalysis, and physiological role, Physiol. Revs.50, 319–375 (1970).Google Scholar