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, Volume 41, Supplement 1, pp C97–C98 | Cite as

Inhibition of anaphylactic shock by gadolinium chloride-induced Kupffer cell blockade

  • G. LázárJr.
  • G. Lázár
  • J. Kaszaki
  • J. Oláh
  • I. Kiss
  • E. Husztik
Histamine and Diverse Cell Types

Abstract

Data in the literature concerning the role of macrophages in anaphylaxis are contradictory. In the present study, the effect of macrophage blockade induced by gadolinium chloride (GdCl3) on anaphylactic shock is investigated. Our observations show that GdCl3 prevents lethal anaphylactic shock in mice sensitized to ovalbumin. Gadolinium chloride given i.v. in a dose of 1 mg/100 g body weight 24 or 48 h before the elicitation of anaphylactic shock resulted in 80% survival, compared with the 43% survival in the control group. The same dose of this rare-earth metal salt also greatly reduced the mortality in mice sensitized with ovalbumin containingBordetella pertussis vaccine, and similarly abrogated the symptoms of anaphylaxis, including the accumulation of serotonin and histamine in the liver. The results suggest that macrophages play an important role in mouse anaphylaxis.

Keywords

Chloride Body Weight Serotonin Histamine Gadolinium 

References

  1. [1]
    J. R. Border,Hypothesis: sepsis, multiple organ failure, and macrophages. Editorial. Arch. Surg.123, 285–286 (1988).PubMedGoogle Scholar
  2. [2]
    E. Husztik, G. Lázár and Á. Párducz,Electron microscopic study of Kupffer cell phagocytosis blockade induced by gadolinium chloride. Br. J. Exp. Path.61, 624–630 (1980).Google Scholar
  3. [3]
    G. Lázár, M. van Galen and G. L. Scherphof,Gadolinium chloride-induced shift in intrahepatic distributions of liposomes. Biochim. Biophys. Acta1011, 97–101 (1989).PubMedGoogle Scholar
  4. [4]
    M. K. Shellenberger and J. H. Gordon,A rapid simplified procedure for simultaneous assay of norepinephrine, dopamine and 5-hydroxytryptamine from brain areas. Anal. Biochem.39, 356–365 (1971).PubMedGoogle Scholar
  5. [5]
    M. A. Beaven and Z. Horakova,The enzymatic isotopic assay of histamine. InHandbook of Experimental Pharmacology. (Ed. M. Roche e Silva) Vol. 18, pp. 151–173, Springer, Berlin 1978.Google Scholar
  6. [6]
    M. Yamaga and C. H. Evans,Suppression of mitogen- and antigen-induced lymphocyte proliferation by lanthanides. Experientia45, 1129–1131 (1989).PubMedGoogle Scholar
  7. [7]
    B. Jenei, G. Lázár, K. Bartha and G. A. Medgyesi,Hypotensive action of IgG preparations containing aggregates suppressed by gadolinium chloride (an agent blocking Kupffer cell function). Agents and Actions32, 333–338 (1992).Google Scholar
  8. [8]
    M. J. Hardonk, F. W. Dijkhuis, C. E. Hulstaert and J. Koudstaal,Heterogeneity of rat liver and spleen macrophages in gadolinium chloride-induced elimination and repopulation. J. Leukoc. Biol.52, 296–302 (1992).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1994

Authors and Affiliations

  • G. LázárJr.
    • 1
  • G. Lázár
    • 2
  • J. Kaszaki
    • 3
  • J. Oláh
    • 4
  • I. Kiss
    • 2
  • E. Husztik
    • 2
  1. 1.Department of SurgeryAlbert Szent-Györgyi Medical UniversitySzegedHungary
  2. 2.Institute of PathophysiologyAlbert Szent-Györgyi Medical UniversitySzegedHungary
  3. 3.Institute of Experimental SurgeryAlbert Szent-Györgyi Medical UniversitySzegedHungary
  4. 4.Department of DermatologyAlbert Szent-Györgyi Medical UniversitySzegedHungary

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