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, Volume 41, Supplement 1, pp C28–C29 | Cite as

Functional comparison of different histamine-containing IgE-receptor positive cells

  • J. Böttjer
  • U. Amon
  • H. H. Wolff
Histamine Release and Mast Cells


The present study was performed to investigate the histamine-releasing activity of non-immunological stimuli on cultured mast cell lines in comparison to isolated skin mast cells and basophils as human therapeutic target cells. The ionophore A23187 induced a dose dependent histamine release from all cell populations (enzymatically isolated human skin mast cells, human peripheral basophils and rat basophilic leukemia cells, RBL-1 and RBL-2H3). The lectin concanavalin A and the tripeptide formyl-methionyl-leucyl-phenylalanine activated only basophils, while the neural mediator substance P and compound 48/80 were active only in experiments with skin mast cells. Activators of protein kinase C (different phorbol esters and the non-phorbol mezerein) induced direct histamine release only from basophils. The data provide further evidence for heterogencity of mast cells and indicate different signal transduction mechanisms following non-immunological activation.


Mast Cell A23187 Histamine Release Signal Transduction Mechanism Mast Cell Line 


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Copyright information

© Birkhäuser Verlag 1994

Authors and Affiliations

  • J. Böttjer
    • 1
  • U. Amon
    • 1
  • H. H. Wolff
    • 1
  1. 1.Department of DermatologyMedical University of LübeckLübeckGermany

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