Agents and Actions

, Volume 41, Supplement 1, pp C28–C29 | Cite as

Functional comparison of different histamine-containing IgE-receptor positive cells

  • J. Böttjer
  • U. Amon
  • H. H. Wolff
Histamine Release and Mast Cells

Abstract

The present study was performed to investigate the histamine-releasing activity of non-immunological stimuli on cultured mast cell lines in comparison to isolated skin mast cells and basophils as human therapeutic target cells. The ionophore A23187 induced a dose dependent histamine release from all cell populations (enzymatically isolated human skin mast cells, human peripheral basophils and rat basophilic leukemia cells, RBL-1 and RBL-2H3). The lectin concanavalin A and the tripeptide formyl-methionyl-leucyl-phenylalanine activated only basophils, while the neural mediator substance P and compound 48/80 were active only in experiments with skin mast cells. Activators of protein kinase C (different phorbol esters and the non-phorbol mezerein) induced direct histamine release only from basophils. The data provide further evidence for heterogencity of mast cells and indicate different signal transduction mechanisms following non-immunological activation.

Keywords

Mast Cell A23187 Histamine Release Signal Transduction Mechanism Mast Cell Line 

References

  1. [1]
    U. Amon, E. v. Stebut, K. R. Dietz, F. W. Bauer and H. H. Wolff,Pharmacological studies on the role of protein kinase C in signal transduction of human basophils. Int. Arch. Allergy Appl. Immunol.98, 349–354 (1992).Google Scholar
  2. [2]
    T. Hultsch, J. L. Rodriguez, M. A. Kaliner and R. J. Hohman,Cyclosporin A inhibits degranulation of rat basophilic leukemia cells and human basophils. Inhibition of mediator release without affecting PI hydrolysis or Ca 2+ fluxes. J. Immunol.144, 2659–2664 (1989).Google Scholar
  3. [3]
    U. Amon and K. R. Dietz,Protein kinase C: A target for antiinflammatory therapy. Arch. Dermatol. Res.284, 8–10 (1992).PubMedGoogle Scholar
  4. [4]
    K. Ozawa, Z. Szallasi, M. G. Kazanietz, P. M. Blumberg, H. Mischak, J. F. Mushinski and M. A. Beaven,Ca 2+-dependent and Ca 2+-independent isozymes of protein kinase C mediate exocytosis in antigen-stimulated rat basophilic RBL-2H3 cells. J. Biol. Chem.268, 1749–1756 (1993).PubMedGoogle Scholar
  5. [5]
    J. C. Foreman, C. C. Jordan, P. Oehme and H. Renner,Structure-activity relationships for some substance P-related peptides that cause wheal and flare reactions in human skin. J. Physiol.335, 449–465 (1983).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1994

Authors and Affiliations

  • J. Böttjer
    • 1
  • U. Amon
    • 1
  • H. H. Wolff
    • 1
  1. 1.Department of DermatologyMedical University of LübeckLübeckGermany

Personalised recommendations