Functional comparison of different histamine-containing IgE-receptor positive cells
- 18 Downloads
The present study was performed to investigate the histamine-releasing activity of non-immunological stimuli on cultured mast cell lines in comparison to isolated skin mast cells and basophils as human therapeutic target cells. The ionophore A23187 induced a dose dependent histamine release from all cell populations (enzymatically isolated human skin mast cells, human peripheral basophils and rat basophilic leukemia cells, RBL-1 and RBL-2H3). The lectin concanavalin A and the tripeptide formyl-methionyl-leucyl-phenylalanine activated only basophils, while the neural mediator substance P and compound 48/80 were active only in experiments with skin mast cells. Activators of protein kinase C (different phorbol esters and the non-phorbol mezerein) induced direct histamine release only from basophils. The data provide further evidence for heterogencity of mast cells and indicate different signal transduction mechanisms following non-immunological activation.
KeywordsMast Cell A23187 Histamine Release Signal Transduction Mechanism Mast Cell Line
- U. Amon, E. v. Stebut, K. R. Dietz, F. W. Bauer and H. H. Wolff,Pharmacological studies on the role of protein kinase C in signal transduction of human basophils. Int. Arch. Allergy Appl. Immunol.98, 349–354 (1992).Google Scholar
- T. Hultsch, J. L. Rodriguez, M. A. Kaliner and R. J. Hohman,Cyclosporin A inhibits degranulation of rat basophilic leukemia cells and human basophils. Inhibition of mediator release without affecting PI hydrolysis or Ca 2+ fluxes. J. Immunol.144, 2659–2664 (1989).Google Scholar