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, Volume 41, Supplement 1, pp C28–C29 | Cite as

Functional comparison of different histamine-containing IgE-receptor positive cells

  • J. Böttjer
  • U. Amon
  • H. H. Wolff
Histamine Release and Mast Cells

Abstract

The present study was performed to investigate the histamine-releasing activity of non-immunological stimuli on cultured mast cell lines in comparison to isolated skin mast cells and basophils as human therapeutic target cells. The ionophore A23187 induced a dose dependent histamine release from all cell populations (enzymatically isolated human skin mast cells, human peripheral basophils and rat basophilic leukemia cells, RBL-1 and RBL-2H3). The lectin concanavalin A and the tripeptide formyl-methionyl-leucyl-phenylalanine activated only basophils, while the neural mediator substance P and compound 48/80 were active only in experiments with skin mast cells. Activators of protein kinase C (different phorbol esters and the non-phorbol mezerein) induced direct histamine release only from basophils. The data provide further evidence for heterogencity of mast cells and indicate different signal transduction mechanisms following non-immunological activation.

Keywords

Mast Cell A23187 Histamine Release Signal Transduction Mechanism Mast Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Birkhäuser Verlag 1994

Authors and Affiliations

  • J. Böttjer
    • 1
  • U. Amon
    • 1
  • H. H. Wolff
    • 1
  1. 1.Department of DermatologyMedical University of LübeckLübeckGermany

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