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Inhibitory effect of interleukin-2 on histamine release from rat mast cells

Agents and Actions volume 41, pages C26–C27 (1994)Cite this article

Abstract

Interleukin-2 (IL-2) inhibited histamine release from rat mast cells induced by compound 48/80 in a concentration-dependent manner. The inhibitory effect of IL-2 on histamine release was also dependent on the length of the incubation period; the maximum inhibition was achieved at 8 h after IL-2 addition. Furthermore, IL-2 inhibited not only IP3 production but also45Ca uptake in mast cells stimulated by compound 48/80. Since IL-2 enhanced [3H]-leucine uptake into mast cells, this suggests that protein synthesis may be related in some way with the inhibition of histamine release. IL-2 treatment augmented the synthesis of a protein having a molecular weight of approximately 35 kDa. From Western blotting analysis, it became clear that the production of lipocortin-I was augmented in rat mast cells by IL-2 treatment. The present study shows that IL-2 induces the synthesis of lipocortin-I in mast cells and that lipocortin-I may play some role in inhibiting histamine release from mast cells.

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Affiliations

  1. Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, 700, Okayama, Japan

    K. Tasaka, M. Hamada & M. Mio

Authors
  1. K. Tasaka
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  2. M. Hamada
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  3. M. Mio
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Tasaka, K., Hamada, M. & Mio, M. Inhibitory effect of interleukin-2 on histamine release from rat mast cells. Agents and Actions 41, C26–C27 (1994). https://doi.org/10.1007/BF02007751

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  • DOI: https://doi.org/10.1007/BF02007751

Keywords

  • Molecular Weight
  • Western Blotting
  • Blotting Analysis
  • Western Blotting Analysis
  • Protein Synthesis