Summary
Unstirred layers of water complicate the measurement of water permeability across epithelia. In the toad urinary bladder, the hormone vasopressin increases the osmotic water permeability of the granular epithelial cell's luminal membrane, and also leads to the appearance of aggregates of particles within this membrane. The aggregates appear to be markers for luminal membrane osmotic water permeability. This report analyzes the relationship between transbladder osmotic water flow and aggregate frequency, and demonstrates that flow across the bladder is significantly attenuated by unstirred layers of water or by structural barriers other than the luminal membrane when the luminal membrane is made permeable by vasopressin. This analysis in addition yields unique values for the permeabilities of both the luminal membrane and the barriers to water flow which lie in series with it.
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References
Bentley, P.J. 1958. The effects of neurohypophyseal extracts on water transfer across the wall of the isolated urinary bladder of the toad,Bufo marinus. Endocrinology 17:201–209
Brown, D., Grosso, A., deSousa, R.C. 1980. Isoproterenol-induced intramembranous particle aggregation and water flux in toad epidermis.Biochim. Biophys. Acta 596:158–164
Chevalier, J., Bourguet, J., Hugon, J.S. 1974. Membrane associated particles: Distribution in frog urinary bladder epithelium at rest and after oxytocin treatment.Cell Tissue Res. 152:129–140
Diamond, J.M. 1979. Osmotic water flow in leaky epithelia.J. Membrane Biol. 51:195–216
DiBona, D.R., Civan, M.M., Leaf, A. 1969. The cellular specificity of the effect of vasopressin on toad urinary bladder.J. Membrane Biol. 1:79–91
Harmanci, M.C., Kachadorian, W.A., Valtin, H., DiScala, V.A. 1978. Antidiuretic hormone-induced intramembranous particle alterations in mammalian collecting ducts.Am. J. Physiol. 235:F440-F443
Hays, R.M. 1972. The movement of water across vasopressin-sensitive epithelia.Curr. Top. Membr. Transp. 3:339–366
Hays, R.M., Leaf, A. 1962. Studies on the movement of water through the isolated toad bladder and its modification by vasopressin.J. Gen. Physiol. 45:905–919
Kachadorian, W.A., Casey, C., DiScala, V.A. 1978. Time course of ADH-induced intramembranous particle aggregation in toad urinary bladder.Am. J. Physiol. 234:F461-F465
Kachadorian, W.A., Muller, J., Rudich, S.W., DiScala, V.A. 1979. Temperature dependence of ADH-induced water flow and intramembranous particle aggregates in toad bladder.Science 205:910–913
Kachadorian, W.A., Levine, S.D., Wade, J.B., DiScala, V.A., Hays, R.M. 1977. Relationship of aggregated intramembranous particles to water permeability in vasopressin-treated toad urinary bladder.J. Clin. Invest. 59:576–581
Kachadorian, W.A., Wade, J.B., DiScala, V.A. 1975. Vasopressin: Induced structural change in toad bladder luminal membrane.Science 190:67–69
Levine, S.D., Kachadorian, W.A., Verna, N.C., Schlondorff, D. 1980. The effect of hydrazine on transport in toad urinary bladder.Am. J. Physiol. 239:F319-F327
Peachey, L.D., Rasmussen, H. 1961. Structure of the toad's urinary bladder as related to its physiology.J. Biophys. Biochem. Cytol. 10:529–553
Schafer, J.A., Patlak, C.S., Andreoli, T.E. 1974. Osmosis in cortical collecting tubules.J. Gen. Physiol. 64:201–227
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Levine, S.D., Kachadorian, W.A. Barriers to water flow in vasopressin-treated toad urinary bladder. J. Membrain Biol. 61, 135–139 (1981). https://doi.org/10.1007/BF02007640
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DOI: https://doi.org/10.1007/BF02007640