Evaluation of a commercial latex test for serological diagnosis ofHelicobacter pylori infection in treated and untreated patients
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The value of a commercially available latex test (Pyloriset) for the diagnosis ofHelicobacter pylori infection by demonstration of specific antibodies was compared with that of direct diagnostic methods such as culture, biopsy-urease test and microscopy of fuchsin-stained smears. The sera were from 136 patients who prior to this study either had or had not been treated forHelicobacter pylori-infection simultaneously with amoxicillin (3 × 750 mg/day) and metronidazole (3 × 500 mg/day) for 12 days. On average, the sensitivity of the test was 90 %. The specificity with sera from untreated patients was 75.9 %; with sera from treated patients specificity was 22.2 %, 28 % and 20 % 1, 3 and 6 months respectively after start of treatment. Only as late as one year after the onset of chemotherapy did the specificity return to 67 %. Because of its low specificity this test does not offer any advantage over other tests in the detection ofHelicobacter pylori-infection or in monitoring the chemotherapeutic success.
KeywordsInternal Medicine Specific Antibody Metronidazole Amoxicillin Pylorus Infection
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- 2.Hirschl AM Frequency of occurrence ofCampylobacter pylori and analysis of the systemic and local immune response. Zentralblatt für Bakteriologie (A) 1987, 266: 526–542.Google Scholar
- 3.Hirschl AM, Pletschette M, Hirschl MH, Berger J, Stanek G, Rotter ML Comparison of different antigen preparations in an evaluation of the immune response toCampylobacter pylori. European Journal of Clinical Microbiology and Infectious Diseases 1988, 7: 570–575.Google Scholar
- 5.Jacobs E, Apel I, Kist M, Bredt W Antibody response of patients against a 120 KDa surface protein ofCampylobacter pylori. Zentralblatt für Bakteriologie (A) 1988, 268: 271–276.Google Scholar
- 6.Loffeld RJLF, Stobberingh E, Flendrig JA, Van Spreeuwel JP, Arends JW Diagnostic value of an immunoassay to detect anti-Campylobacter pylori antibodies in non-ulcer dyspepsia. Lancet 1989, i: 1182–1185.Google Scholar
- 9.Newell DG, Rathbone BJ The serodiagnosis ofCampylobacter pylori infection. Serodiagnosis Immunotherapy 1989, 3: 1–6.Google Scholar
- 10.Newell DG, Stacey AR The serology ofCampylobacter pylori infection. In: Rathbone BJ, Heatley RV (ed):Campylobacter pylori and gastroduodenal disease. Blackwell Scientific Publications, Oxford, 1989, p. 74–82.Google Scholar
- 12.Stacey AR, Hawtin PR, Newell DG The antigenicity of fractions ofHelicobacter pylori prepared by fast liquid chromatography and urease captured by monoclonal antibodies. European Journal of Clinical Microbiology and Infectious Diseases 1990, 9: 732–737.Google Scholar
- 13.Dent JC, McNulty CAM Evaluation of a new selective medium forCampylobacter pylori. European Journal of Clinical Microbiology and Infectious Diseases 1988, 7: 555–558.Google Scholar
- 14.Hirschl AM, Rotter ML Serodiagnosis ofHelicobacter pylori infections: suitability of various antigen preparations. In: Malfertheiner P, Ditschuneit H (ed):Helicobacter pylori gastritis and peptic ulcer. Springer Verlag, Berlin, 1990, p. 141–146.Google Scholar
- 15.Rolke J, Börsch G, Geis G, Mai U, Opferkuch W Die Serodiagnostik vonCampylobacter pylori. Immunität und Infektion 1989, 17: 78–82.Google Scholar
- 17.Gobert B, Bene MC, de Korwin JD, Faure D Isotype evolution in the follow-up study of patients withCampylobacter pylori associated gastritis. Gastroenterologic Clinique et Biologique 1989, 13: 880–883.Google Scholar