Summary
Antibodies reacting with 3,5,3′,5′ tetraiodo-l-tyrosyl-l-tyrosine (I2Tyr-I2Tyr) were elicited in rabbits by immunization with an oxidized yeast conjugate coupled with I2Tyr-I2Tyr. Ion-exchange chromatography was used to purify immunoglobulins, in order to improve the specificity in measurement of I2Tyr-I2Tyr level in patient serum. IgG binding capacity versus I2Tyr-I2Tyr was considerably increased after immunoglobulin purification.
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Acknowledgments. The authors with to thank Mrs M. Ollier for her valuable technical assistance.
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Bernard, D., Maurizis, J.C., Nicolas, C. et al. IgG purification to measure the level of an iodinated thyroglobulin peptide, the 3,5,3′,5′ tetraiodo-l-tyrosyl-l-tyrosine in human serum. Experientia 41, 385–387 (1985). https://doi.org/10.1007/BF02004519
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DOI: https://doi.org/10.1007/BF02004519