Skip to main content
Log in

A new plot to estimate protein molecular weight by density gradient ultracentrifugation

  • Published:
Experientia Aims and scope Submit manuscript

Summary

A plot of the logarithm of the molecular weight against the logarithm of the sedimenting distance is proposed for estimation of protein molecular weight. The proteins are separated in acrylamide-containing linear density gradients, polymerized and stained after centrifugation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Martin, R. G., and Ames, B. N., J. biol. Chem.236 (1961) 1372.

    Article  CAS  PubMed  Google Scholar 

  2. Jolley, W. B., Allen, H. W., and Griffith, O. M., Analyt. Biochem.21 (1967) 454.

    Article  CAS  PubMed  Google Scholar 

  3. Calcagno, M., Diaz-Zagoya, J. C., and Gascón, A., Analyt. Biochem.73 (1976) 386.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rendón, J.L., Calcagno, M. A new plot to estimate protein molecular weight by density gradient ultracentrifugation. Experientia 41, 382–383 (1985). https://doi.org/10.1007/BF02004517

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02004517

Key words

Navigation