Summary
A plot of the logarithm of the molecular weight against the logarithm of the sedimenting distance is proposed for estimation of protein molecular weight. The proteins are separated in acrylamide-containing linear density gradients, polymerized and stained after centrifugation.
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References
Martin, R. G., and Ames, B. N., J. biol. Chem.236 (1961) 1372.
Jolley, W. B., Allen, H. W., and Griffith, O. M., Analyt. Biochem.21 (1967) 454.
Calcagno, M., Diaz-Zagoya, J. C., and Gascón, A., Analyt. Biochem.73 (1976) 386.
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Rendón, J.L., Calcagno, M. A new plot to estimate protein molecular weight by density gradient ultracentrifugation. Experientia 41, 382–383 (1985). https://doi.org/10.1007/BF02004517
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DOI: https://doi.org/10.1007/BF02004517