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Further studies on gel filtration of urinary substances

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  • Chimica, Biochimica
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Summary

Urine specimens from healthy children were concentrated and filtered through Sephadex G-100 and G-25. The eluate was recovered in fractions of 5 ml each. Total hexoses, galactose, total proteins and total uronic acids were determined on each fraction. Total hexoses and galactose were eluted in 6 distinct peaks. The uronic acid containing GAG were eluted in 4 small peaks on G-25 and 1 peak on G-100. This peak is eluted always with high amounts of galactose containing glycoproteins.

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Author notes

  1. J. A. Kofoed (Member of the ‘Carrera del Investigador Cientifico’)

    Present address: Consejo Nacional de Investigaciones Cientificas y Técnicas, Argentina

Authors and Affiliations

  1. Catedra de Fisiologia, Facultad de Odontologia, Universidad de Buenos Aires, M. T. de Alvear 2142, Buenos Aires, Argentina

    J. A. Kofoed (Member of the ‘Carrera del Investigador Cientifico’), A. C. Barcelo & A. A. Tocci

Authors
  1. J. A. Kofoed
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  2. A. C. Barcelo
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  3. A. A. Tocci
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Additional information

This work was partially supported by research grants from the CONICET, República Argentina.

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Kofoed, J.A., Barcelo, A.C. & Tocci, A.A. Further studies on gel filtration of urinary substances. Experientia 32, 851–852 (1976). https://doi.org/10.1007/BF02003724

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  • DOI: https://doi.org/10.1007/BF02003724

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