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Glycoprotein biosynthesis in splenic cells. Purification of a microsomal galactosyl-transferase

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  • Chimica, Biochimica
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Summary

One part of the microsomal galactosyl-transferase activity of splenic cells of rats can by solubilized by the action of Triton X-100 and Tween 20. Its purification on a Sephadex G-200 column and by electrophoresis on a polyacrylamide gel leads to a solution of high specific enzymic activity.

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Literatur

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Author information

Authors and Affiliations

  1. Lyon-Sud Medical School, Laboratory of Biochemistry and E.R.A. CNRS No. 562, University of Lyon, B.P. 12, F-69600, Oullins, France

    A. Martin, M. Richard & P. Louisot

Authors
  1. A. Martin
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  2. M. Richard
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  3. P. Louisot
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Additional information

This work has benefited from the help of the ‘Centre National de la Recherche Scientifique’, the ‘Direction des Recherches et Moyens d'Essais’, the ‘Institut National de la Santé et de la Recherche Médicale’, the ‘Fondation pour la Recherche Médicale Française’ the ‘Délégation Générale à la Recherche Scientifique et Technique’ and the ‘Université de Lyon (UER Lyon-Sud et Biologie Humaine’).

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Martin, A., Richard, M. & Louisot, P. Glycoprotein biosynthesis in splenic cells. Purification of a microsomal galactosyl-transferase. Experientia 32, 844–846 (1976). https://doi.org/10.1007/BF02003720

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  • DOI: https://doi.org/10.1007/BF02003720

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