Copper and aspirin treatment increase the antioxidant activity of plasma
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Copper (Cu) complexes of a number of compounds have increased anti-inflammatory efficacy over the native compound. In the present study, Cu and acetylsalicylic acid (ASA) were administered to rabbits over a one week period. At the end of this time, ocular inflammation was induced by intravitreal injection of endotoxin. ASA caused a slight reduction in the inflammatory response at 24 hours, but this response was not further decreased when Cu was added to the treatment regimen. However, there was a gradual and significant increase in antioxidant activity in the plasma of animals in the combined treatment group which could not be accounted for by a Cu-ASA complex, an increase in the concentration of known plasma protein antioxidants (ceruloplasmin and transferrin) or an increase in superoxide dismutase activity. The compound(s) responsible for this increased activity has a molecular weight greater than 10 000 Da. A large increase in plasma antioxidant activity may have a protective effect in other models of inflammation and provide a mechanism for the proven increase in anti-inflammatory efficacy of Cu-chelates of many compounds.
KeywordsSuperoxide Aspirin Antioxidant Activity Superoxide Dismutase Plasma Protein
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- A. Conforti, L. Franco, R. Milanino and G. P. Velo,Copper and ceruloplasmin (Cp) concentrations during the acute inflammatory process in the rat. Agent and Actions12, 303–307 (1982).Google Scholar
- D. R. Blake, N. D. Hall, D. A. Treby, B. Halliwell and J. M. C. Gutteridge,Oxygen free radicals and lipid peroxidation inhibition by the protein ceruloplasmin. FEBS Lett.112, 269–274 (1980).Google Scholar
- R. Milanino and G. P. Velo,Multiple actions of copper in control of inflammation: studies in copper-deficient rats. Agents and Actions [Suppl.]8, 209–228 (1977).Google Scholar
- M. C. McGahan, L. N. Fleisher and A. M. Grimes,Copper depletion and d-penicillamine treatment alter some parameters of the ocular inflammatory response. Invest. Opthalmol. Vis. Sci. (Suppl.)29, 287 (1988).Google Scholar
- J. R. J. Sorenson,Some copper coordination compounds and their anti-inflammatory and anti-ulcer activities. Inflammation1, 317–325 (1976a).Google Scholar
- D. H. Brown, W. E. Smith, J. W. Teape and A. J. Lewis,Anti-inflammatory effects of some copper complexes. J. Med. Chem.23, 792–732 (1980).Google Scholar
- E. J. Lewis and G. B. West,Copper complexes and experimental-induced inflammation and arthritis, InTrace Elements in the pathogenesis and treatment of inflammation. (Eds. K. D. Rainsford and M. W. Whitehouse) pp 339–358, Birkhauser 1981).Google Scholar
- M. Rister, K. Bauermeister, U. Gravert and E. Gladtke,Superoxide deficiency in rheumatoid arthritis. Lancet, May 20, 1094 (1978).Google Scholar
- J. R. J. Sorenson,Copper complexes: a physiologic approach to treatment of chronic diseases. Comp. Ther.11, 49–64 (1985).Google Scholar
- M. C. McGahan, L. N. Fleisher and A. M. Grimes,Ferroxidase activity increases dramatically in the aqueous humor during the ocular inflammatory reponse. Opthal. Res.21, 221–225 (1989).Google Scholar