Skip to main content
SpringerLink
Log in

A simplified assay for cyclic AMP using protein kinase binding

  • Specialia
  • Published:
Experientia Aims and scope Submit manuscript

Summary

The protein kinase binding assay for cAMP was modified by substitution of adsorption by QAE cellulose for the membrane filtration. This modification obviates the variation of recovery of cAMP with the volume of buffer used to wash the filter. The assay is reproducible and technically simpler than those currently employed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. A. G. Gilman, Proc. Nat. Acad. Sci. USA.67, 305 (1970).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. B. L. Brown, J. D. M. Albano, R. P. Ekins, A. M. Sgherzi and W. Tampion, Biochem. J.121, 561 (1971).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. G. M. Walton and L. D. Garren, Biochemistry9, 4223 (1970).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Hematology-Oncology Section, Department of Medicine, Hospital of the University of Pennsylvania, 19104, Philadelphia, PA, USA

    A. K. Sinha & R. W. Colman

Authors
  1. A. K. Sinha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. W. Colman
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Acknowledgment. This work was partially supported by grants HL-16583 and HL-18827 from the National Heart and Lung Institute.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sinha, A.K., Colman, R.W. A simplified assay for cyclic AMP using protein kinase binding. Experientia 33, 301–302 (1977). https://doi.org/10.1007/BF02002791

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02002791

Keywords

Search

Navigation