The effects of acetylation on the binding region of cartilage proteoglycans to hyaluronic acid
- 23 Downloads
Proteoglycans in cartilage are found as aggregates and as monomers. Evidence has been obtained indicating that hyaluronic acid, normally present in this tissue, binds together monomers into large molecular weight aggregates. In this investigation, the interacting region of the protein backbone has been studied. The results unequivocally demonstrated that the epsilon amino groups of lysine are involved in hyaluronic acid binding to proteoglycans and that their blocking by acetylation either prevents reaggregation or disaggregates the high mol. w aggregates.
KeywordsMolecular Weight Lysine Hyaluronic Acid Interact Region Binding Region
- 4.D. Heinegärd and V. C. Hascall, in: Protides of Biological Fluids, 22nd Colloquium, p. 177. Pergamon Press 1974.Google Scholar
- 5.T. E. Hardingham and H. Muir, Biochem. J.195, 905 (1973).Google Scholar
- 6.J. D. Gregory, in: Protides of Biological Fluids, 22nd Colloquium, p. 171. Pergamon Press 1974.Google Scholar
- 7.Abbreviation. A1 proteoglycan aggregates; A1D1, proteoglycan subunits or monomers; HA, hyaluronic acid; GuHcl, guanidinium hydrochloride; TRU, turbidimetric units.Google Scholar