Experientia

, Volume 33, Issue 3, pp 298–299 | Cite as

The effects of acetylation on the binding region of cartilage proteoglycans to hyaluronic acid

  • G. Quintarelli
  • A. Vocaturo
  • R. Zito
  • L. Rodén
Specialia
  • 22 Downloads

Summary

Proteoglycans in cartilage are found as aggregates and as monomers. Evidence has been obtained indicating that hyaluronic acid, normally present in this tissue, binds together monomers into large molecular weight aggregates. In this investigation, the interacting region of the protein backbone has been studied. The results unequivocally demonstrated that the epsilon amino groups of lysine are involved in hyaluronic acid binding to proteoglycans and that their blocking by acetylation either prevents reaggregation or disaggregates the high mol. w aggregates.

Keywords

Molecular Weight Lysine Hyaluronic Acid Interact Region Binding Region 

Literatur

  1. 2.
    D. Heinegärd and V. C. Hascall, J. biol. Chem.249, 4250 (1974).PubMedGoogle Scholar
  2. 3.
    T. E. Hardingham and H. Muir, Biochim. biophys. Acta279, 401 (1972).PubMedGoogle Scholar
  3. 4.
    D. Heinegärd and V. C. Hascall, in: Protides of Biological Fluids, 22nd Colloquium, p. 177. Pergamon Press 1974.Google Scholar
  4. 5.
    T. E. Hardingham and H. Muir, Biochem. J.195, 905 (1973).Google Scholar
  5. 6.
    J. D. Gregory, in: Protides of Biological Fluids, 22nd Colloquium, p. 171. Pergamon Press 1974.Google Scholar
  6. 7.
    Abbreviation. A1 proteoglycan aggregates; A1D1, proteoglycan subunits or monomers; HA, hyaluronic acid; GuHcl, guanidinium hydrochloride; TRU, turbidimetric units.Google Scholar
  7. 8.
    G. Quintarelli, A. Vocaturo, M. Bellocci, L. Rodén, E. Ippolito and J. R. Baker, Am. J. Anat.140, 433 (1974).PubMedGoogle Scholar
  8. 9.
    T. Bitter and H. Muir, Analyt. Biochem.4, 330 (1962).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1977

Authors and Affiliations

  • G. Quintarelli
    • 1
    • 2
    • 3
  • A. Vocaturo
    • 1
    • 2
    • 3
  • R. Zito
    • 1
    • 2
    • 3
  • L. Rodén
    • 1
    • 2
    • 3
  1. 1.The Regina Elena Institute for Cancer ResearchRoma(Italy)
  2. 2.Department of PathologyUniversity of Alabama in Birmingham(USA)
  3. 3.Institute for Dental Research, School of Dentistry and Departments of Medicine and BiochemistryUniversity of Alabama in Birmingham(USA)

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