Annals of Vascular Surgery

, Volume 7, Issue 5, pp 488–490 | Cite as

Endothelial seeding of vascular prostheses: A technique of in situ enzymatic retrieval of endothelial cells without vein sacrifice

  • Joel Combe
  • Jean-Michel Buniet
  • Patrick Hervé
  • Gabriel Camelot
Selected Techniques


The success of endothelial seeding of vascular prosthetic grafts is largely dependent on endothelial cell retrieval. An in situ cell harvesting technique using enzymatic dissolution in a temporarily excluded segment of the internal jugular vein is described. With this technique the cellular yield is adequate and the internal jugular vein remains patent. This technique allows for high-density seeding in a single stage. In two-stage seeding the excellent yield of cells reduces the duration of in vitro endothelial cell culture and allows for secondary massive and early seeding.


Endothelial Cell Cell Culture Abdominal Surgery Internal Jugular Vein Single Stage 
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Copyright information

© Annals of Vascular Surgery Inc. 1993

Authors and Affiliations

  • Joel Combe
    • 1
  • Jean-Michel Buniet
    • 1
  • Patrick Hervé
    • 1
  • Gabriel Camelot
    • 1
  1. 1.From the Service de Chirurgie Vasculaire and Unité de Recherche du Centre Régional de Transfusion SanguineCHU Jean MinjozBesançonFrance

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