Basic Research in Cardiology

, Volume 75, Issue 1, pp 66–72 | Cite as

Prospects of X-ray microanalysis in the study of pathophysiology of myocardial contraction

  • M. F. Wendt-Gallitelli
  • H. Wolburg
  • W. Schlote
  • M. Schwegler
  • C. Holubarsch
  • R. Jacob
Article

Summary

X-ray microanalysis was used to compare chemically untreated cryosections of quick-frozen myocardial tissue in “caffeine contracture” with cryosections of normal muscle. Our goal was to find out if it is possible by means of this method to detect changes in the calcium compartmentalization of the myocardial cell occurring by changes in its functional state. While it is possible to quantitate calcium in the cisternae of sarcoplasmic reticulum of the control muscle preparation, calcium could never be detected in these compartments of caffeine-contracted muscles.

In active microsomal fraction of ventricular myocardium it is possible to quantitate calcium and also to distinguish two components on account of their different ability to accumulate this element. The calcium content is different in the two components of the fraction.

Keywords

Calcium Caffeine Functional State Sarcoplasmic Reticulum Calcium Content 

Perspektiven der Röntgenmikroanalyse für Untersuchungen über die Pathophysiologie der myokardialen Kontraktion

Zusammenfassung

Ultradünne Kryoschnitte von chemisch unbehandeltem, schnell gefrorenem Myokardgewebe in Koffein-Kontraktur wurden verglichen mit Kontrollpräparaten mit der Methode der Röntgenmikroanalyse. Während in den Kontrollpräparaten Calcium in den Regionen nachweisbar ist, die den terminalen Zysternen des sarkoplasmatischen Retikulums entsprechen, ist dieses Element in den Kontrakturexperimenten nie in diesen Regionen nachweisbar. Häufig dagegen tritt dieses Element in den Mitochondrien auf.

In aktiven mikrosomalen Fraktionen aus dem Ventrikelmyokard ist es auch möglich, Calcium zu quantifizieren. Zwei Komponenten können in der Fraktion unterschieden werden, die sich auf Grund ihrer Fähigkeit, Calcium zu akkumulieren, unterscheiden. Der Calciumgehalt in den Komponenten kann bestimmt werden.

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Copyright information

© Dr. Dietrich Steinkopff Verlag 1980

Authors and Affiliations

  • M. F. Wendt-Gallitelli
    • 1
  • H. Wolburg
    • 1
  • W. Schlote
    • 1
  • M. Schwegler
    • 1
  • C. Holubarsch
    • 1
  • R. Jacob
    • 1
  1. 1.Physiologisches Institut II, Institut für Submikroskopische PathologieUniversität TübingenTübingen

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