Skip to main content
SpringerLink
Log in

Circumventing the diapause of potato cyst nematodes

  • Published:
Netherlands Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

The diapause of potato cyst nematodes was bypassed by avoiding desiccation of the cysts. Larvae were artificially hatched by cutting the cysts in halves and subsequent incubation in potato root diffusate. Approximately 40% of the cyst content hatched. These treatments had no influence on viability and fecundity as ascertained by rearing nematodes in pots and on roots of sprouts grown on water agar in Petri dishes. With the artificial hatching procedure it is possible to produce five to six generations a year in Petri dishes and three to five generations in pots.

Samenvatting

De diapauze van aardappelcysteaaltjes kan worden omzeild door te voorkómen dat de cysten uitdrogen. Hiertoe worden de cysten opgekweekt op wortels van aard-appelspruiten in Petrischalen met wateragar of in potten en zorgvuldig vochtig gehouden.

De larven worden uit de eieren gelokt door de cysten met een scalpel te halveren of zorgvuldig door te drukken zonder de eieren te beschadigen en deze vervolgens te incuberen in lokstof. Op deze wijze wordt ongeveer 40% van de cysteïnhoud gelokt. Deze behandeling heeft geen nadelige invloeden op de vitaliteit van de larven en de vrucht-baarheid van de hieruit ontwikkelde mannetjes en vrouwtjes. Dit geldt zowel voor eieren uit cysten opgekweekt in Petrischalen als die in potten.

Op deze wijze is het mogelijk drie tot vijf generaties per jaar in potten te kweken en vijf tot zes generaties in Petrischalen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Clark, A.J. & Perry, R.N., 1977. Hatching of cyst-nematodes. Nematologica 23: 350–368.

    Article  Google Scholar 

  • Cunningham, P.C., 1960. An investigation of winter dormancy inHeterodera rostochiensis. Scientific Proceedings of the Royal Dublin Society 1: 1–4.

    Google Scholar 

  • Ellenby, C. & Smith, L., 1967. Emergence of larvae from the new cysts of the potato-root eelworm,Heterodera rostochiensis. Nematologica 13: 273–278.

    Article  Google Scholar 

  • Green, C.D., Greet, D.N. & Jones, F.G.W., 1970. The influence of multiple mating on the reproduction and genetics ofHeterodera rostochiensis andH. schachtii. Nematologica 16: 309–326.

    Article  Google Scholar 

  • Guile, C.T., 1967. On cyst colour changes, bionomics and distribution of potato cyst-eelworm (Heterodera rostochiensis Woll.) pathotypes in the East Midlands. Annals of Applied Biology 60: 411–419.

    Google Scholar 

  • Kort, J., 1960. A technique for the extraction ofHeterodera cysts from wet soil and for the estimation of their eggs and larval content. Mededelingen Plantenziektenkundige Dienst Wageningen no. 233: 3–7.

    Google Scholar 

  • Kort, J., Ross, H., Rumpenhorst, H.J. & Stone, A.R., 1977. An international scheme for identifying and classifying pathotypes of potato cyst-nematodesGlobodera rostochiensis andG. pallida. Nematologica 23: 333–339.

    Article  Google Scholar 

  • McKenna, L.A. & Winslow, R.D., 1972. Differences in hatch and development rates of potato cyst nematode pathotypes. Annals of Applied Biology 71: 274–278.

    Google Scholar 

  • Mugniéry, D., 1982. A method for screening potatoes for resistances toGlobodera sp. under laboratory conditions. In: Hooker, W.J. (Ed.), Research for the potato in the year 2000. CIP, p. 137–138.

  • Mugniéry, D. & Fayet, G., 1984. Détermination du sexe deGlobodera rostochiensis Woll. et influence des niveaux d'infestation sur la pénétration, le développement et le sexe de ce nématode. Revue de Nématologie 7: 233–238.

    Google Scholar 

  • Mugniéry, D. & Person, F., 1976. Méthode d'élevage de quelques nématodes à kystes du genreHeterodera. Sciences Agronomiques Rennes, 217–220.

  • Oostenbrink, M., 1960. Estimating nematode populations by some selected methods. In: Sasser, J.N. & Jenkins, W.R. (Eds), Nematology. Chapel Hill, Univ. of North Carolina Press, p. 85–102.

    Google Scholar 

  • Rode, H., 1971. Der Einfluss verschiedener Temperaturen und Wechselreize auf das Schlüpfen von Larven des Kartoffelnematoden. Pedobiologia 11: 143–158.

    Google Scholar 

  • Shepherd, A.M. & Cox, P.M., 1967. Observations on periodicity of hatching of eggs of the potato cyst nematode,Heterodera rostochiensis Woll. Annals of Applied Biology 60: 143–150.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Nematology, Agricultural University, Binnenhaven 10, 6709 PD, Wageningen, the Netherlands

    R. Janssen, J. Bakker & F. J. Gommers

Authors
  1. R. Janssen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Bakker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. J. Gommers
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Janssen, R., Bakker, J. & Gommers, F.J. Circumventing the diapause of potato cyst nematodes. Netherlands Journal of Plant Pathology 93, 107–113 (1987). https://doi.org/10.1007/BF02000561

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02000561

Additional keywords

Search

Navigation