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, Volume 39, Issue 3–4, pp 97–103 | Cite as

HRF of 30 kDa: Evidence for active synthesis

  • Chantal Brunet
  • Jacques Hébert
Allergy and Histamine

Abstract

A variety of mediators are involved in the pathophysiology of a number of clinical conditions. Among them histamine-releasing factors (HRF) act as secretagogue for basophils and mast cells to cause cell degranulation and histamine release. Studies on the kinetics of HRF production by activated mononuclear cells (MNC) have suggested an active synthesis of this cytokine. Using a [35S]-metabolic cell labeling method, we first analyzed the capacity of MNC from patients with allergic rhinitis to ragweed to actively synthesize HRF. We then found that stimulation of MNC with allergen promoted a synthesis of large quantities of proteins, including a protein of about 30 kDa. Secondly, we developed a method for the enrichment of HRF molecules from crude supernatants of ragweed-stimulated MNC using ion exchange and gel filtration chromatography. We observed that the yield of HRF activity is seen just before the chymotrypsinogen marker. The estimated molecular weight was 30–35 kDa.

Keywords

Molecular Weight Mast Cell Histamine Mononuclear Cell Clinical Condition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • Chantal Brunet
    • 1
  • Jacques Hébert
    • 1
  1. 1.Centre de Recherche en Inflammation et Immunologie-RhumatologieLe Centre Hospitalier de l'Université LavalSainte-FoyCanada

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