The Journal of Membrane Biology

, Volume 62, Issue 3, pp 231–237 | Cite as

Amino acid-dependent sodium transport in plasma membrane vesicles from rat liver

  • Herman J. Sips
  • Karel van Dam


Thel-alanine-dependent transport of sodium ions across the plasma membrane of rat-liver parenchymal cells was studied using isolated plasma membrane vesicles. Sodium uptake is stimulated specifically by thel-isomer of alanine and other amino acids, whose transport is sodium-dependent in rat-liver plasma membrane vesicles. Thel-alanine-dependent sodium flux across the membrane is inhibited by an excess of Li+ ions, but not by K+ or choline ions. Sodium transport is sensitive to-SH reagents and ionophores, and is an electrogenic process: a membrane potential (negative inside) can enhancel-alanine-dependent sodium accumulation. The data presented provide further evidence for a sodium-alanine cotransport mechanism.

Key words

Plasma membrane rat liver sodium transport 


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Copyright information

© Springer-Verlag New York Inc. 1981

Authors and Affiliations

  • Herman J. Sips
    • 1
  • Karel van Dam
    • 1
  1. 1.Laboratory of Biochemistry, B.C.P. Jansen InstituteUniversity of AmsterdamAmsterdamThe Netherlands

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