The Journal of Membrane Biology

, Volume 62, Issue 3, pp 207–218 | Cite as

Excitation-revealed changes in cytoplasmic Cl concentration in “Cl-starved”Chara cells

  • M. J. Beilby


The changes in the cytoplasmic Cl concentration, [Cl] c , are monitored at the time of withdrawal (starvation) and subsequent replacement of Cl in the outside medium. The measurement technique exploits the involvement of Cl inChara excitation. The transient clamp current due to Cl,ICl, is separated from other excitation transients through Hodgkin-Huxley (HH) equations, which have been adjusted toChara. TheICl amplitude depends on HH parameters, [Cl] c and the maximum membrane conductance to Cl,\(\overline {g_{Cl} } \). The results are discussed in terms of these quantities.ICl and\(\overline {g_{Cl} } \) were found to fall after 6–10 hr of Cl starvation, thus supporting the hypothesis that [Cl c decreases in Cl-free medium. The best HH fit to “starved” data was obtained with [Cl c =3.5mm. The time-course forICl decline is considerably slower than the time-course of the rise of the starvation-stimulated influx. As cells starved for periods longer than 24 hr are re-exposed to Cl, it is revealed that while [Cl] c remains low during long starvation,\(\overline {g_{Cl} } \) increases to values greater than those of the normal cells. Such differences among cells starved for various lengths of time have not been detected previously.

Key words

Chara voltage clamp cytoplasm action potential Cl concentration Cl transport Cl starvation 


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Copyright information

© Springer-Verlag New York Inc. 1981

Authors and Affiliations

  • M. J. Beilby
    • 1
  1. 1.Botany SchoolCambridgeEngland

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