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Netherlands Journal of Plant Pathology

, Volume 94, Issue 6, pp 307–310 | Cite as

Use of detached tomato leaves to test for resistance to tomato mosaic virus

  • Pim Lindhout
  • Wanda Korta
  • Jeanne Dijkstra
Short Communication

Samenvatting

Een methode werd ontwikkeld om de resistentie van in de kas geteelde tomateplanten tegen tomatemozaïek virus (ToMV) te bepalen. Bladeren van een vatbare en een resistente cultivar werden afgesneden en geïnoculeerd met ToMV. Na 6, 10 en 17 dagen werden de geïnoculeerde bladeren getoetst op de aanwezigheid van virus met ELISA en door inoculatie van bladeren vanNicotiana glutinosa. Met beide toetsmethoden kon de virustoename in de vatbare cultivar al vroeg na inoculatie duidelijk worden aangetoond. In de bladeren van de resistente cultivar was een zeer kleine hoeveelheid virus pas laat na de inoculatie aantoonbaar. Met deze methode is het mogelijk om de resistentie tegen ToMV te bepalen, tevens zaad te winnen en landbouwkundige eigenschappen te evalueren, zonder de plant te infecteren.

Additional keywords

ELISA bioassy Lycopersicon esculentum 

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1988

Authors and Affiliations

  • Pim Lindhout
    • 1
  • Wanda Korta
    • 2
  • Jeanne Dijkstra
    • 3
  1. 1.Institute for Horticultural Plant Breeding (IVT)Wageningenthe Netherlands
  2. 2.Station for Horticultural Plant BreedingKrzeszowicePoland
  3. 3.Department of Virology, WageningenAgricultural UniversityWageningenthe Netherlands

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