Agents and Actions

, Volume 31, Issue 3–4, pp 257–262 | Cite as

Calcium mobilization in activated mast cells monitored by flow cytometric analysis

  • G. L. Rossi
  • D. J. Young
  • S. I. Wasserman
  • K. E. Barrett
Allergy, Histamine und Kinins


Changes in intracellular calcium levels induced in mast cells by either antigen or ionomycin were monitored using flow cytometry and the calcium binding dye indo-1. Both stimuli increased calcium levels in responsive cells by a similar amount, but not all cells examined were responsive to antigen. Antigen-induced increases in intracellular calcium levels could be completely blocked by the calcium antagonist TMB-8. Flow cytometry appears to be a useful method for monitoring mast cell calcium concentrations, as it provides the capability to study large numbers of individual cells.


Calcium Flow Cytometry Mast Cell Calcium Concentration Individual Cell 
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Copyright information

© Birkhäuser Verlag 1990

Authors and Affiliations

  • G. L. Rossi
    • 1
  • D. J. Young
    • 2
  • S. I. Wasserman
    • 1
  • K. E. Barrett
    • 1
  1. 1.Division of Allergic and Immunologic Diseases, Department of MedicineUniversity of California, San Diego School of MedicineSan DiegoUSA
  2. 2.Division of Rheumatology, Department of MedicineUniversity of California San Diego School of MedicineSan DiegoUSA

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