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Agents and Actions

, Volume 31, Issue 3–4, pp 257–262 | Cite as

Calcium mobilization in activated mast cells monitored by flow cytometric analysis

  • G. L. Rossi
  • D. J. Young
  • S. I. Wasserman
  • K. E. Barrett
Allergy, Histamine und Kinins

Abstract

Changes in intracellular calcium levels induced in mast cells by either antigen or ionomycin were monitored using flow cytometry and the calcium binding dye indo-1. Both stimuli increased calcium levels in responsive cells by a similar amount, but not all cells examined were responsive to antigen. Antigen-induced increases in intracellular calcium levels could be completely blocked by the calcium antagonist TMB-8. Flow cytometry appears to be a useful method for monitoring mast cell calcium concentrations, as it provides the capability to study large numbers of individual cells.

Keywords

Calcium Flow Cytometry Mast Cell Calcium Concentration Individual Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    M. A. Beaven, J. Rogers, J. P. Moore, T. R. Hesketh, G. A. Smith and J. C. Metcalfe,The mechanism of the calcium signal and correlation with histamine release in 2H3 cells. J. Biol. Chem.259, 7129–7136 (1984).PubMedGoogle Scholar
  2. [2]
    J. R. White, T. Ishizaka and R. I. Sha'afi,Direct demonstration of increased intracellular concentration of free calcium as measured by Quin-2 in stimulated rat peritoneal mast cell. Proc. Natl. Acad. Sci. USA81, 3978–3982 (1984).PubMedGoogle Scholar
  3. [3]
    P. J. Millard, D. Gross, W. W. Webb and C. Fewtrell,Imaging asynchronous changes in intracellular Ca ++ in individual stimulated tumor mast cells. Proc. Natl. Acad. Sci. USA85, 1854–1858 (1988).PubMedGoogle Scholar
  4. [4]
    P. S. Rabinovitch, C. H. June, A. Grossman and J. A. Ledbetter,Heterogeneity among T cells in intracellular free calcium responses after mitogen stimulation with PHA or anti-CD3. Simultaneous use of indo-1 and immunofluorescence with flow cytometry. J. Immunol.137, 952–961 (1986).PubMedGoogle Scholar
  5. [5]
    A. W. Griffioen, G. T. Rijkers, J. Keij and B. J. M. Zegers,Measurement of cytoplasmic calcium in lymphocytes using flow cytometry. J. Immunol. Meth.120, 23–27 (1989).CrossRefGoogle Scholar
  6. [6]
    A. Pezzutto, P. S. Rabinovitch, B. Dorken, G. Moldenhauer and E. A. Clark,Role of the CD22 human B cell antigen in B cell triggering by anti-immunoglobulin. J. Immunol.140, 1791–1795 (1988).PubMedGoogle Scholar
  7. [7]
    T. A. Davies, D. Drotts, G. J. Weil and E. R. Simons,Flow cytometric measurements of cytoplasmic calcium changes in human platelets. Cytometry9, 138–142 (1988).CrossRefPubMedGoogle Scholar
  8. [8]
    R. Y. Tsien, T. Pozzan and T. J. Rink,Calcium homeostasis in intact lymphocytes: cytoplasmic calcium monitored with a new intracellularly trapped fluorescence dye. J. Cell Biol.94, 325–334 (1982).PubMedGoogle Scholar
  9. [9]
    N. Chand, W. Diamantis, J. Pillar and R. D. Sofia,Inhibition of allergic and non-allergic histamine secretion from rat peritoneal mast cells by calcium antagonists. Brit. J. Pharmacol.83, 899–902 (1984).Google Scholar
  10. [10]
    D. L. Marquardt and L. L. Walker,Inhibition of mast cell adenosine responsiveness by chronic exposure to adenosine receptor agonists. Biochem. Pharmacol.36, 4297–4302 (1987).CrossRefPubMedGoogle Scholar
  11. [11]
    K. G. Lazzari, P. J. Proto and E. R. Simons,Simultaneous measurement of stimulus-induced changes in cytoplasmic Ca ++ and in membrane potential in human neutrophils. J. Biol. Chem.261, 9710–9713 (1986).PubMedGoogle Scholar
  12. [12]
    N. Chakravarty,Effect of TMB-8 on calcium transport in mast cells in relation to histamine secretion. Agents and Actions23, 191–193 (1988).PubMedGoogle Scholar
  13. [13]
    N. Grosman,Effect of TMB-8 on histamine release from isolated rat mast cells. Int. Archs. Allergy Appl. Immunol.79, 253–258 (1986).Google Scholar

Copyright information

© Birkhäuser Verlag 1990

Authors and Affiliations

  • G. L. Rossi
    • 1
  • D. J. Young
    • 2
  • S. I. Wasserman
    • 1
  • K. E. Barrett
    • 1
  1. 1.Division of Allergic and Immunologic Diseases, Department of MedicineUniversity of California, San Diego School of MedicineSan DiegoUSA
  2. 2.Division of Rheumatology, Department of MedicineUniversity of California San Diego School of MedicineSan DiegoUSA

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