Plant Cell, Tissue and Organ Culture

, Volume 33, Issue 1, pp 81–89 | Cite as

Effect of cytokinins on plastid development and photosynthetic polypeptides during organogenesis ofPinus ponderosa Dougl. cotyledons culturedin vitro

  • Alicia Mazari
  • Edith L. Camm
Original Papers

Abstract

InPinus ponderosa Dougl., application of the cytokinins, benzyladenine and 2-isopentenyl adenine, to excised cotyledons, promoted thein vitro formation of meristematic centers which led to bud and shoot production. Meristematic cells showed plastids with poorly developed thylakoid membranes and rudimentary grana, whereas cells in non-meristematic tissues and in growth regulator free medium, had chloroplasts with well developed inner membranes, and more thylakoid membranes and grana than plastids of meristematic cells. Chlorophyll and six polypeptides associated with photosynthesis were present in lower concentrations in cytokinin-treated cotyledons than in those cultured in growth regulator free medium. Both benzyladenine and 2-isopentenyl adenine are effective in inhibiting the accumulation of at least two photosynthetic polypeptides in the first 24 h in culture. The ability of cotyledons to respond in this way to cytokinins is lost after three days in culture in growth regulator free medium prior to treatment with cytokinin.

Key words

cytokinins in vitro organogenesis photosynthetic polypeptides Pinus ponderosa plastid 

Abbreviations

BA

benzyladenine

CF1

coupling factor

CK

cytokinin

33EP

33kD extrinsic polypeptide

GRF

growth regulator free

2iP

2-isopentenyl adenine

LSU-RUBP

large subunit of Ribulose-1,5-bisphosphate carboxylase

LHCI

light harvesting complex of photosystem I

LHCII

light harvesting complex of photosystem II

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Alicia Mazari
    • 1
  • Edith L. Camm
    • 1
  1. 1.Department of BotanyThe University of British ColumbiaVancouverCanada

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