Abstract
Phosphoenolpyruvate carboxylase (PEPCase) and sucrose phosphate synthase (SPS) were active in nucellar calli of ‘Hamlin’ sweet orange,Citrus sinensis (L.) Osbeck, grown on media containing either 5% sucrose or 2% glycerol as the primary carbon source. Activities of the enzymes, however, were much higher in the glycerol-grown tissues than those grown in sucrose. Glycerol, in addition, stimulated embryogenesis and chlorophyll biosynthesis and initiated the onset of ribulose bisphosphate carboxylase-oxygenase (Rubisco) activity. These stimulatory effects, which were not observed in sucrose-grown calli, became more obvious when calli grown on glycerol-containing, agar-solidified medium were transferred and grown in liquid suspension medium containing 2% glycerol. Starch levels in both sucrose- and glycerol-grown tissues were as high as 30% of tissue dry weight. There were, however, higher concentrations of soluble sugars in sucrose-grown calli than those grown on glycerol. Stimulation of embryogenesis, chlorophyll synthesis, Rubisco onset, and activities of PEPCase and SPS by glycerol offered potential prospects in using this compound in citrus tissue cultures.
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Abbreviations
- PEPCase:
-
phosphoenolpyruvate carboxylase
- Rubisco:
-
ribulose bisphosphate carboxylase-oxygenase
- SPS:
-
sucrose phosphate synthase
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Vu, J.C.V., Niedz, R.P. & Yelenosky, G. Glycerol stimulation of chlorophyll synthesis, embryogenesis, and carboxylation and sucrose metabolism enzymes in nucellar callus of ‘Hamlin’ sweet orange. Plant Cell Tiss Organ Cult 33, 75–80 (1993). https://doi.org/10.1007/BF01997601
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DOI: https://doi.org/10.1007/BF01997601