Advertisement

Agents and Actions

, Volume 35, Issue 3–4, pp 163–169 | Cite as

The role of histamine1 and histamine2 receptors in the ethanol-induced jejunal plasma protein loss

  • D. J. Leddin
  • P. K. Dinda
  • I. T. Beck
Allergy, Histamine and Kinins

Abstract

Histamine and other mediators have been shown to be involved in the ethanol-induced jejunal plasma protein loss. In this study we have investigated whether the histamine (H)-related component of this protein loss is mediated by H1-receptors, H2-receptors or both. Four groups of dogs (n=12 in each) were studied. They were: untreated, H1+H2-receptor blockade, H1-receptor blockade and H2-receptor blockade. Chlorpheniramine and ranitidine were used to block H1 and H2-receptor blockade. Chlorpheniramine and ranitidine were used to block H1 and H2-receptors respectively. In all animals, jejunal protein loss was measured over 10 min periods for 90 min. Ethanol increased protein loss in all time periods (p<0.001). This protein loss was depressed by H1+H2-receptors blockade throughout 90 min (p<0.01). H1-receptor blockade caused a similar depression of ethanol effect but only during 20 to 40 min (p<0.05). In contrast, H2-receptor blockade aggravated the protein losing effect of ethanol throughout 90 min (p<0.01). Analyses of data tend to suggest that the ethanol-induced protein loss is mediated principally by H1-receptors, and that a complete inhibition of the histamine-related ethanol-induced protein loss can be achieved only by a simultaneous blockade of both H1 and H2- receptors, and not by H1- or H2-receptor blockade alone.

Keywords

Histamine Plasma Protein Ranitidine Complete Inhibition Histamine2 Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    M. G. Buell and I. T. Beck,Ethanol-induced mucosal microvascular stasis and enhanced plasma protein loss in the dog jejunum. Gastroenterology86, 413–420 (1984).PubMedGoogle Scholar
  2. [2]
    I. T. Beck, G. P. Morris and M. G. Buell,Ethanol-induced vascular permeability changes in the jejunal mucosa of the dog. Gastroenterology90, 1137–1145 (1986).PubMedGoogle Scholar
  3. [3]
    P. R. Kvietys, W. G. Patterson, J. M. Russell, J. A. Barrowman and D. N. Granger,Role of the microcirculation in ethanol-induced mucosal injury in the dog. Gastroenterology87, 562–571 (1984).PubMedGoogle Scholar
  4. [4]
    D. J. Leddin, M. Ray, P. K. Dinda, I. Prokopiw and I. T. Beck,16,16-dimethyl prostaglandin E 2 alleviates jejunal microvascular effects of ethanol but not the ethanol-induced inhibition of water, sodium and glucose absorption. Gastroenterology94, 726–732 (1988).PubMedGoogle Scholar
  5. [5]
    M. Ray, P. K. Dinda and I. T. Beck,Mechanism of ethanol-induced jejunal microvascular and morphologic changes in the dog. Gastroenterology96, 345–354 (1989).PubMedGoogle Scholar
  6. [6]
    P. K. Dinda, D. J. Leddin and I. T. Beck,Histamine is involved in ethanol-induced jejunal microvascular injury in rabbits. Gastroenterology95, 1227–1233 (1988).Google Scholar
  7. [7]
    I. T. Beck, A. Boyd and P. K. Dinda,Evidence for the involvement of 5-lipoxygenase products in the ethanol-induced intestinal plasma protein loss. Am. J. Physiol.254, G483-G488 (1988).PubMedGoogle Scholar
  8. [8]
    S. T. Nielsen,Characterization of the H 2 receptor antagonist and gastric acid antisecretory properties of Wy-45,727. J. Pharmacol. Exp. Ther.242, 607–613 (1987).PubMedGoogle Scholar
  9. [9]
    M. Somogyi,Determination of blood sugar. J. Biol. Chem.160, 69–73 (1945).Google Scholar
  10. [10]
    Y. Israel, J. G. Valenzuela, I. Salazar and G. Ugarte,Alcohol and amino acid transport in the human small intestine. J. Nutr.98, 222–224 (1969).PubMedGoogle Scholar
  11. [11]
    C. H. Halsted, E. A. Robles and E. Mezey,Distribution of ethanol in the human gastrointestinal tract. Am. J. Clin. Nutr.16, 831–834 (1973).Google Scholar
  12. [12]
    M. S. Millan, G. P. Morris, I. T. Beck and J. T. Henson,Villous damage induced by suction biopsy and by acute ethanol intake in normal human intestine. Dig. Dis. Sci.25, 513–525 (1980).PubMedGoogle Scholar
  13. [13]
    M. G. Buell and I. T. Beck,Effect of ethanol on regional blood flow in the rabbit. Gastroenterology84, 81–89 (1983).PubMedGoogle Scholar
  14. [14]
    M. G. Buell, P. K. Dinda and I. T. Beck,Effect of ethanol on morphology and total, capillary and shunted blood flow of the different anatomical layers of the dog jejunum. Dig. Dis. Sci.28, 1005–1017 (1983).PubMedGoogle Scholar
  15. [15]
    I. T. Beck, P. K. Dinda, D. J. Leddin, M. Ray, I. Prokopiw and A. Boyd,Chemical mediators in the ethanol-induced increased jejunal microvascular permeability. InMicrocirculation in circulatory disorders. (Eds. H. Manabe, B. W. Zweifach and K. Messmer) pp. 171–178, Springer-Verlag Tokyo, Inc., Tokyo 1988.Google Scholar
  16. [16]
    N. A. Mortillaro, D. N. Granger, P. R. Kvietys, G. Rutili and A. E. Taylor,Effects of histamine and histamine antagonists on intestinal capillary permeability. Am. J. Physiol.240, G381-G386 (1981).PubMedGoogle Scholar
  17. [17]
    H. Nagata and P. H. Guth,Effect of topical histamine on mucosal microvascular permeability and acid secretion in the rat stomach. Am. J. Physiol.246, G654-G659 (1984).PubMedGoogle Scholar
  18. [18]
    P. J. Oates and J. P. Hakkinen,Studies on the mechanism of ethanol induced gastric damage in rats. Gastroenterology94, 10–21 (1988).PubMedGoogle Scholar
  19. [19]
    D. A. A. Owen, E. Poy and D. F. Woodward,Evaluation of the role of histamine H 1 and H2 receptors in cutaneous inflammation in the guinea pig produced by histamine and mast cell degranulation. Br. J. Pharmacol.69, 615–623 (1980).PubMedGoogle Scholar
  20. [20]
    J. Kusche, W. Lorenz, C.-D. Stahlknecht, H. Richter, R. Hesterberg, A. Schmal, E. Hinterlang, D. Weber and C. Ohmann,Intestinal diamine oxidase and histamine release in rabbit mesenteric ischemia. Gastroenterology80, 980–987 (1981).PubMedGoogle Scholar
  21. [21]
    B. M. Altura and S. Halevy,Benefical and detrimental actions of histamine H 1- and H2-receptor antagonists in circulatory shock. Proc. Natl. Acad. Sci.75, 2941–2944 (1978).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1992

Authors and Affiliations

  • D. J. Leddin
    • 1
  • P. K. Dinda
    • 1
  • I. T. Beck
    • 1
  1. 1.The Gastrointestinal Diseases Research Unit, Departments of Medicine and PhysiologyQueen's University, Hotel Dieu HospitalKingstonCanada

Personalised recommendations