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, Volume 36, Supplement 2, pp C260–C264 | Cite as

Cytokines modulate IgE-mediated histamine liberation from human basophils

A comparative study of IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, TNF, LT, IFNα, IFNγ, GM-CSF and M-CSF
  • K. S. Kristensen
  • K. Bendtzen
  • S. Norn
  • P. Stahl Skov
  • M. Pedersen
  • H. Permin
Plenary Lecture Histamine Release

Abstract

The effect of recombinant human IL-1α, IL-1β, IL-2, IL-3, Il-4, IL-6, TNF, LT, IFNα, IFNγ, GM-CSF and M-CSF on human basophil histamine release induced by anti-IgE antibody was examined in seven healthy donors using washed whole blood and a glass microfibre assay. The blood was preincubated for 20 min with cytokines and incubated for another 60 min after addition of the antibody. IL-3, GM-CSF and M-CSF were found to enhance the IgE-mediated response by 60–80%, whereas the other cytokines did not affect the mediator release to any significant degree.

Keywords

Histamine Healthy Donor Histamine Release Significant Degree Mediator Release 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1992

Authors and Affiliations

  • K. S. Kristensen
    • 1
  • K. Bendtzen
    • 2
  • S. Norn
    • 3
  • P. Stahl Skov
    • 3
  • M. Pedersen
    • 4
  • H. Permin
    • 4
  1. 1.Department of PharmacologyUniversity of CopenhagenCopenhagenDenmark
  2. 2.Laboratory of Medical ImmunologyState University HospitalCopenhagen
  3. 3.The Reference LaboratoryCopenhagen
  4. 4.University Clinic for Infectious DiseasesState University HospitalCopenhagen

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