Agents and Actions

, Volume 36, Supplement 2, pp C260–C264 | Cite as

Cytokines modulate IgE-mediated histamine liberation from human basophils

A comparative study of IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, TNF, LT, IFNα, IFNγ, GM-CSF and M-CSF
  • K. S. Kristensen
  • K. Bendtzen
  • S. Norn
  • P. Stahl Skov
  • M. Pedersen
  • H. Permin
Plenary Lecture Histamine Release


The effect of recombinant human IL-1α, IL-1β, IL-2, IL-3, Il-4, IL-6, TNF, LT, IFNα, IFNγ, GM-CSF and M-CSF on human basophil histamine release induced by anti-IgE antibody was examined in seven healthy donors using washed whole blood and a glass microfibre assay. The blood was preincubated for 20 min with cytokines and incubated for another 60 min after addition of the antibody. IL-3, GM-CSF and M-CSF were found to enhance the IgE-mediated response by 60–80%, whereas the other cytokines did not affect the mediator release to any significant degree.


Histamine Healthy Donor Histamine Release Significant Degree Mediator Release 
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Copyright information

© Birkhäuser Verlag 1992

Authors and Affiliations

  • K. S. Kristensen
    • 1
  • K. Bendtzen
    • 2
  • S. Norn
    • 3
  • P. Stahl Skov
    • 3
  • M. Pedersen
    • 4
  • H. Permin
    • 4
  1. 1.Department of PharmacologyUniversity of CopenhagenCopenhagenDenmark
  2. 2.Laboratory of Medical ImmunologyState University HospitalCopenhagen
  3. 3.The Reference LaboratoryCopenhagen
  4. 4.University Clinic for Infectious DiseasesState University HospitalCopenhagen

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