Cytokines modulate IgE-mediated histamine liberation from human basophils
The effect of recombinant human IL-1α, IL-1β, IL-2, IL-3, Il-4, IL-6, TNF, LT, IFNα, IFNγ, GM-CSF and M-CSF on human basophil histamine release induced by anti-IgE antibody was examined in seven healthy donors using washed whole blood and a glass microfibre assay. The blood was preincubated for 20 min with cytokines and incubated for another 60 min after addition of the antibody. IL-3, GM-CSF and M-CSF were found to enhance the IgE-mediated response by 60–80%, whereas the other cytokines did not affect the mediator release to any significant degree.
KeywordsHistamine Healthy Donor Histamine Release Significant Degree Mediator Release
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