Abstract
Mast cells from infant foreskin obtained during circumcision were dispersed by an enzymatic technique, pooled, washed and purified. Mast cells, with a purity of 70–90% were incubated with 10−11 to 10−7 M rTNFα. Histamine and tryptase levels were assessed in the cell supernatant and a concentration dependent release of histamine was observed. Histamine reached a maximum of 11.5±2.2 nmol/106 cells at 10−8 M rTNF. Tryptase reached a maximum of 293±105 mU/106 cells (10−8 M rTNF). rTNFα thus appears to be a direct stimulus for mast cells to degranulate and to release both histamine and tryptase.
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van Overveld, F.J., Jorens, P.G., Rampart, M. et al. Tumor necrosis factor — a novel stimulus for human skin mast cells to secrete histamine and tryptase. Agents and Actions 36 (Suppl 2), C256–C259 (1992). https://doi.org/10.1007/BF01997345
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DOI: https://doi.org/10.1007/BF01997345