Abstract
Twenty-three penicillinase-producing strains ofNeisseria gonorrhoeae were characterized. All strains showed hybridization with a TEM-1 probe and production of a TEM-1 type beta-lactamase (pI 5.4) on isoelectric focusing. Eight strains also showed a beta-lactamase band of pI 5.5. The penicillin MICs for the strains producing only TEM-1 were in the range 4–16 µg/ml; MICs of the strains with the additional pI 5.5 band were≥128 µg/ml for seven strains and 4 µg/ml for an arginine-hypoxanthine dependent strain. The association of the high MICs with the presence of the pI 5.5 band was statistically significant. The pI 5.5 band could represent a new beta-lactamase type or a TEM-1 mutant.
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References
Ashford WA, Golash RG, Heming VG Penicillinase-producingNeisseria gonorrhoeae. Lancet 1976, ii: 657–658.
Wiedemann B, Kliebe C, Kresken M The epidemiology of β-lactamases. Journal of Antimicrobial Chemotherapy 1989, 24, Supplement B: 1–22.
Genco CA, Knapp JS, Clark VL Conjugation of plasmids ofNeisseria gonorrhoeae to otherNeisseria species: potential reservoirs for the β-lactamase plasmid. Journal of Infectious Diseases 1984, 150: 397–401.
Baró T, García Conesa J, Vázquez JA, Miralles R, Alia C Estudio de la sensibilidad antimicrobiana, auxotipia y análisis plasmídico de 75 cepas deNeisseria gonorrhoeae aisladas en Barcelona. Medicina Clínica 1989, 92: 765–768.
Matthew M, Harris AM, Marshall ML, Ross GW The use of analytical isoelectric focusing for detection and identification of beta-lactamases. Journal of General Microbiology 1975, 88: 169–178.
Sanders CC, Sanders WE, Moland ES Characterization of β-lactamases in situ on poliacrylamide gels. Antimicrobial Agents and Chemotherapy 1986, 30: 951–952.
Hermida M, Gibert I, Barbé J, Roy C Estudio comparativo con los marcadores digoxigenina y biotina para la identificación mediante sondas de ADN de los genes que codifican betalactamasas de tipo TEM. Enfermedades Infecciosas y Microbiología Clínica 1990, 8: 501–504.
National Committee for Clinical Laboratory Standards Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard M7-A2. 2nd Edition. NCCLS, Villanova, PA, 1990.
Catlin BW Characteristics and auxotyping ofNeisseria gonorrhoeae. In: Bergan T, Norris JR (ed): Methods in microbiology. Volume 10. Academic Press, London, 1978, p. 345–380.
Kado CL, Liu ST Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 1981, 145: 1365–1373.
Knapp JS, Holmes KK Disseminated gonococcal infections caused byNeisseria gonorrhoeae with unique nutritional requirements. Journal of Infectious Diseases 1975, 132: 204–208.
Van Klingeren B, Ansik-Schipper MC, Dessens-Kroon M, Verheuvel M Relationship between auxotype pattern and susceptibility to antibiotics in penicillinase-producingNeisseria gonorrhoeae. Journal of Antimicrobial Chemotherapy 1985, 16: 143–147.
Gouby A, Bourg G, Ramuz M Previously undescribed 6,6 kilobase plasmid in penicillinase-producingNeisseria gonorrhoeae. Antimicrobial Agents and Chemotherapy 1986, 29: 1095–1097.
Matthew M, Harris AM Identification of β-lactamases by analytical isoelectric focusing: correlation with bacterial taxonomy. Journal of General Microbiology 1976, 94: 55–67.
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Hermida, M., Roy, C., Baró, M.T. et al. Characterization of penicillinase-producing strains ofNeisseria gonorrhoeae . Eur. J. Clin. Microbiol. Infect. Dis. 12, 45–48 (1993). https://doi.org/10.1007/BF01997057
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DOI: https://doi.org/10.1007/BF01997057