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Agents and Actions

, Volume 38, Issue 3–4, pp C206–C208 | Cite as

Histamine release by free radicals: Effect of defibrotide (DFT) and its fraction “P.o. 085 D V”

  • F. Gambassi
  • M. Lupini
  • M. G. Di Bello
  • L. Mugnai
  • E. Masini
  • P. F. Mannaioni
Histamine Release from Mast Cells and Basophils

Abstract

The effects of defibrotide (DFT) and its fraction P.O. 085 D V on rat serosal mast cell histamine release evoked by free radicals were studied. Free radical production was obtained with: a) metabolic activation of arachidonic acid and cocaine by means of liver microsomes (S-10 mix) from polychlorinated biphenyl mixture (PCB)-treated rats; b) metabolic activation of arachidonic acid by means of prostaglandin-H-synthetase (PHS) and c) human platelet aggregation induced by arachidonic acid. DFT and its fraction reduced histamine release evoked by aggregating human platelets; P.O. 085 D V significantly reduced histamine release evoked by metabolic activation of both cocaine and arachidonic acid in the presence of S-10 mix, while DFT was less active on the cocaine-S 10 mix system and ineffective on the arachidonic acid-S 10 mix system. Both drugs were unable to decrease histamine release induced by metabolic activation of arachidonic acid in the presence of PHS.

Keywords

Free Radical Cocaine Mast Cell Histamine Arachidonic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. [1]
    R. Niada, M. Mantovani, G. Prinò, R. Pescador, F. Berti, C. Omini and G. C. Folco,Antithrombotic activity of a polydeoxyribonucleotidic substance extracted from mammalian organs: a possible link with prostacyclin. Thromb. Res.23, 233–246 (1981).PubMedGoogle Scholar
  2. [2]
    C. Thiemermann, P. Löbel and K. Schrör,Usefulness of defibrotide in protecting ischaemic myocardium from early reperfusion damage. Am. J. Cardiol.56, 978–982 (1985).PubMedGoogle Scholar
  3. [3]
    M. Lupini, F. Gambassi, L. Mugnai, S. Bianchi, E. Masini and P. F. Mannaioni,Defibrotide decreases histamine release in a guinea-pig model of myocardial ischemia and reperfusion “in vitro”. Agents Actions,Special Conference Issue, C333–C335 (1992).Google Scholar
  4. [4]
    E. Masini, B. Palmerani, F. Gambassi, A. Pistelli, E. Gianella, B. Occupati, M. Ciuffi, T. Bani Sacchi and P. F. Mannaioni,Histamine release from rat mast cells induced by metabolic activation of polyunsaturated fatty acids into free radicals. Biochem. Pharmacol.39, 879–889 (1990).PubMedGoogle Scholar
  5. [5]
    P. F. Mannaioni, E. Giannella, B. Palmerani, A. Pistelli, F. Gambassi, T. Bani-Sacchi, S. Bianchi and E. Masini,Plenary lecture. Free radicals as endogenous releasers. Agents Actions23, 129–142 (1988).PubMedGoogle Scholar
  6. [6]
    P. F. Mannaioni, A. Pistelli, F. Gambassi, M. G. Di Bello, S. Raspanti and E. Masini,A place for free radicals in platelet-derived histamine releasing factor (PDHRF) and evidence that histaminergic receptors modulate platelet aggregation. Agents Actions33, 57–60 (1991).PubMedGoogle Scholar
  7. [7]
    R. C. Garner, E. C. Miller and J. A. Miller,Liver microsomal metabolism of aflatoxin B to a reactive derivative toxic to Salmonella Tiphimurium-TA 1530. Cancer Res.32, 2058–2066 (1972).PubMedGoogle Scholar
  8. [8]
    E. Masini, A. Pistelli, F. Gambassi, M. G. Di Bello and P. F. Mannaioni,Definition of platelet-derived histamine releasing factor (PDHRF) and histaminergic receptors modulating platelet aggregation. Advances in Prostaglandin, Thromboxane, and Leukotriene Research21, 533–536 (1990).Google Scholar

Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • F. Gambassi
    • 1
  • M. Lupini
    • 1
  • M. G. Di Bello
    • 1
  • L. Mugnai
    • 1
  • E. Masini
    • 1
  • P. F. Mannaioni
    • 1
  1. 1.Department of Preclinical and Clinical PharmacologyUniversity of FlorenceFlorenceItaly

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