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Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification, antiserum preparation, serological identification, and detection in pelargonium by ELISA

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Abstract

Two viruses, detected frequently in the Netherlands in pelargonium, were identified by serology and test plant reactions. Antisera were prepared and an ELISA procedure was developed to detect the viruses in pelargonium.

One of the viruses, PFBV-N, proved to be pelargonium flower-break virus. With the antiserum to PFBV-N, it could be detected reliably throughout the year inPelargonium zonale ‘Springtime Irene’.

The other virus, PLPV-N, was serologically closely related to pelargonium line pattern virus (PLPV) and to pelargonium ring pattern virus (PRPV), as were an old virus isolate from ‘Saturnus’, collected in the Netherlands in 1971 (L128), and PLPV isolates from Yugoslavia (PLPV-Y) and Denmark (PLPV-D). There were only minor differences in host-plant reactions between the virus isolates. Based on these tests, PLPV and PRPV are considered as isolates of the same virus, for which, for practical reasons, the name pelargonium line pattern virus is proposed.

PLPV could be reliably detected by ELISA inP. zonale ‘Springtime Irene’ and ‘Amanda’ throughout the year with only a few exceptions. InPelargonium peltatum ‘Tavira’, however, reslts were erratic due to uneven distribution of virus in the plant. Best results were obtained when petioles of fully expanded leaves were tested.

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  1. DLO-Research Institute for Plant Protection (IPO-DLO), P.O. Box 9060, 6700 GW, Wageningen, the Netherlands

    I. Bouwen & D. Z. Maat

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  1. I. Bouwen
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  2. D. Z. Maat
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Bouwen, I., Maat, D.Z. Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification, antiserum preparation, serological identification, and detection in pelargonium by ELISA. Netherlands Journal of Plant Pathology 98, 141–156 (1992). https://doi.org/10.1007/BF01996326

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