Skip to main content
Log in

Fusaric acid: phytotoxicity and in vitro production by Fusarium oxysporum f.sp. lilii, the causal agent of basal rot in lilies

  • Articles
  • Published:
Netherlands Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

The production of phytotoxins byFusarium oxysporum f.sp.lilii, a pathogen causing bulb and scale rot in lilies, was investigated. To determine the toxic activity of culture filtrate, a bioassay with in vitro grown scale bulblets or with callus was set up. The fungus produces toxic components in different culture media. The highest toxicity was observed in Czapek Dox medium. HPLC and GC/MS analyses revealed the presence of fusaric acid at toxic concentrations in this medium. The production of fusaric acid in time coincides with the increase of toxic activity in the culture filtrate. It is concluded that at least part of this toxicity is due to fusaric acid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Arcioni, S., Pezzotti, M. & Damiani, F., 1987. In vitro selection of alfalfa plants resistant toFusarium oxysporum f.sp.medicaginis. Theoretic and Applied Genetics. 74: 700–705.

    Google Scholar 

  • Behnke, M., 1980. Selection of dihaploid potato callus for resistance to the culture filtrate ofFusarium oxysporum. Zeitschrift für Pflanzenzüchtung 85: 254–258.

    Google Scholar 

  • Chawla, H.S. & Wenzel, G., 1987. In vitro selection for fusaric acid resistant barley plants. Plant Breeding 99: 159–163.

    Google Scholar 

  • Dobson, T.A., Desaty, D., Brewer, D. & Vining, L.C., 1967. Biosynthesis of fusaric acid in cultures ofFusarium oxysporum Schlecht. Canadian Journal of Biochemistry 45: 809–823.

    Article  PubMed  CAS  Google Scholar 

  • Drysdale, R.B., 1982. The production and significance in phytopathology of toxins produced by species ofFusarium. In: Moss, M.O. & Smith, J.E. (Eds), The applied mycology of fusarium, p. 95–105.

  • Gengenbach, B.G. & Green, C.E., 1975. Selection of T-cytoplasm maize callus cultures resistant toHelminthosporium maydis race T pathotoxin. Crop Science 15: 645–649.

    Article  Google Scholar 

  • Hartman, C.L., McCoy, T.J. & Knous, T.R., 1984. Selection of alfalfa (Medicago sativa) cell lines and regeneration of plants resistant to the toxin(s) produced byFusarium oxysporum f.sp.medicaginis. Plant Science Letters 34: 183–194.

    Article  Google Scholar 

  • Health-Pagliuso, S. & Rappaport, L., 1990. Somaclonal variant UC-T3: the expression of Fusarium wilt resistance in progeny arrays of celery,Apium graveolens L. Theoretic and Applied Genetics 80: 390–394.

    Google Scholar 

  • Imle, E.P., 1942. Bulb rot diseases of lilies. Lily Yearbook of the American Lily Society, p. 30–41.

  • Jullien, M., 1988. Effects of theFusarium sp. toxins and selection of crude toxin resistant strains in mesophyll cell cultures ofAsparagus officinalis. Plant Physiology and Biochemistry 26: 123–721.

    Google Scholar 

  • Kern, H., 1972. Phytotoxins produced by Fusaria. In: Wood, R.K.S., Ballio, A. & Graniti, A. (Eds), Phytotoxins in Plant Diseases. Academic Press, New York, p. 35–48.

    Google Scholar 

  • Linsmaier, E.M. & Skoog F., 1965. Organic growth factor requirements of tobacco tissue cultures. Physiological Plantarum 18: 100–127.

    CAS  Google Scholar 

  • Löffler, H.J.M. & Mouris, J.R., 1990. Prospects of in vitro selection for resistance againstFusarium oxysporum in lily. In: De Jong (Ed), Proceedings Eucarpia symposium Integration of in vitro techniques in ornamental plant breeding, 10–14 november 1990, Wageningen, p. 80–85.

  • Marasas, W.F.O., Nelson, P.E. & Tousson, T.A., 1984. Toxigenic Fusarium species: Identification and Mycotoxicology. Pennsylvania State University Press, Univ. Park.

    Google Scholar 

  • Matsui, Y. & Watanabe, M., 1988. Quantitative analysis of fusaric acid in the culture filtrate and soybean plants inoculated withFusarium oxysporum var.redolens. Journal of Rakuno Gakuen University 13: 159–167.

    CAS  Google Scholar 

  • Matsui, Y., Murayama, M., Nishi, S. & Ihnuma, A., 1988. Soybean blight caused byFusarium oxysporum var.redolens and the production of fusaric acid by the fungus. Journal of the College of Dairying, 12: 403–412.

    Google Scholar 

  • Mégnégneau, B. & Branchard, M., 1988. Toxicity of fusaric acid observed on callus cultures of variousCucumis melo genotypes. Plant Physiology and Biochemistry 26: 585–588.

    Google Scholar 

  • Ouchi, S., Toyoda, H., Utsumi, R., Hashimoto, H. & Hadama, T., 1989. A promising strategy for the control of fungal disease by the use of toxin-degrading microbes. In: Graniti, A., Durbin, R.D. & Ballio, A. (Eds), Phytotoxins and Plant Pathogenesis. NATO ASI Series vol 27, Springer Verlag, Berlin, p. 301–317.

    Google Scholar 

  • Wenzel, G. & Foroughi-Wehr, B., 1990. Progeny tests of barley, wheat and potato regenerated from cell cultures after in vitro selection for disease resistance. Theoretical and Applied Genetics 80: 359–365.

    Article  Google Scholar 

  • Yoder, O.C., 1980. Toxins in pathogenesis. Annual Review of Phytopathology 18: 103–129.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Löffler, H.J.M., Mouris, J.R. Fusaric acid: phytotoxicity and in vitro production by Fusarium oxysporum f.sp. lilii, the causal agent of basal rot in lilies. Netherlands Journal of Plant Pathology 98, 107–115 (1992). https://doi.org/10.1007/BF01996323

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01996323

Additional keywords

Navigation