Chronic intrajejunal TNBS application in TNBS-sensitized rats: A new model of chronic inflammatory bowel diseases
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An enteritis, based on a delayed-type hypersensitivity reaction, was induced in TNBS (2,4,4-trinitrobenzenesulfonic acid) sensitized rats by intrajejunal challenge with TNBS. This treatment induced a chronic inflammation of the distal small intestine, characterized by gross hyperaemia and oedema, as assessed by a macroscopic score. Histologically, the inflammatory response included cell infiltration by lymphocytes and histiocytes, a transmural granulomatous inflammation with multinucleated cells and activated mesenteric lymph nodes. Drug treatment with sulfasalazine or 5-aminosalicylic acid improved enteritis score. The applicability and relevance of this new model is discussed in relation to drug development and basic research of inflammatory bowel diseases.
KeywordsLymph Node Inflammatory Response Inflammatory Bowel Disease Small Intestine Drug Treatment
- M. M. Aparicio-Pages, H. W. Verspaget, A. S. Pena, I. T. Weterman, P. A. de Bruin, M. A. Mierement-Ooms, J. M. van der Zon, E. A. van Tol and C. B. Lamers,In vitro cellular cytotoxicity in Crohn's disease and ulcerative colitis: Relation with disease activity and treatment, and the effect of recombinant gamma-interferon. J. Clin. Lab. Immunol.29, 119–124 (1989).PubMedGoogle Scholar
- C. Fiocchi,Immune events associated with inflammatory bowel disease. Scand. J. Gastroenterol.25, 4–12 (1990).Google Scholar
- W. Beeken, S. St. Andre-Ukena and R. M. Gundel,Histochemical and cytotoxicity studies of peripheral blood monocytes in Crohn's disease. Gastroenterology78, 1138 (1980).Google Scholar
- J. L. Rabbins and R. S. Cotran (Eds.),The Gastrointestinal Tract. InPathologic Basis of Disease, pp. 95–987 W.B. Saunders Company, Philadelphia, London 1979.Google Scholar