Netherlands Journal of Plant Pathology

, Volume 89, Issue 6, pp 275–281 | Cite as

The genetic and molecular basis of b-proteins in the genus Nicotiana

  • S. Gianinazzi
  • Patricia Ahl
Review Papers


Screening for the pathogenesis-related (b) protein patterns of 11Nicotiana species and 30N. tabacum varieties has revealed both inter- and intraspecific variability and 7 different b-proteins (b0, b1, b1′, b1″, b2, b3 and b4) have been clearly defined. Their genetic determinants are sexually transmitted independently of theN gene conferring resistance to TMV, and a monogenic inheritance has been demonstrated for one of them (b1′). Grafting experiments have revealed the existence of a species-aspecific ‘mobile compound’ responsible for the expression of the b-protein genes, the production of which is probably under the control of theN gene. Among the 5 intraspecific and 6 interspecific hybrids studied, one of them, theN. glutinosa x N. debneyi together with its amphidiploid, synthesizes b-protein (b1″) in a constituve way and possesses a high level of resistance to necrosis-inducing viruses. The amphidiploid is able to transfer these two properties to otherNicotianae not only by crossing but also by grafting; it therefore appears to permanently synthesize the ‘mobile compounds’. Furthermore, the hypersensitive reaction to TMV in these hybrids is only completely broken down at 35 °C, whereas this normally occurs at 30 °C in plants with theN gene.

Additional keywords

interspecific and intraspecific hybrids grafting experiments N gene resistance temperature effects 


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  1. Ahl, P., 1979. Résistance aux virus et protéines b chez le tabac. Travail de diplôme, University of Geneva, Switzerland.Google Scholar
  2. Ahl, P., 1983. Aspects génétiques et moléculaires de la résistance (RH) chez lesNicotiana. Ph D Thesis, University of Geneva, Switzerland.Google Scholar
  3. Ahl, P. & Gianinazzi, S., 1982. b-Proteins as a constitutive component in highly (TMV) resistant interspecific hybrids ofN.glutinosa x N. debneyi. Pl. Sci. Lett. 26: 173–181.CrossRefGoogle Scholar
  4. Ahl, P., Cornu, A. & Gianinazzi, S., 1982. Soluble proteins as genetic markers in studies of resistance and phylogeny inNicotiana. Phytopathology 72: 80–85.CrossRefGoogle Scholar
  5. Ahl, P., Gianinazzi, S. & Cornu, A., 1983. A new potential for enhancing resistance to tobacco mosaic virus inNicotiana species Neth. J. Pl. Path. 89 (No. 6): ... (abstract).Google Scholar
  6. Antoniw, J.F., Ritter, C.E., Pierpoint, W.S. & Loon, L.C. van, 1980. Comparison of three pathogenesis-related proteins from plants of two cultivars of tobacco infected with TMV. J. gen. Virol. 47: 79–87.CrossRefGoogle Scholar
  7. Carr, J.P., Antoniw, J.F., White, R.F. & Wilson, T.M.A., 1982. Latent messenger RNA in tobacco (Nicotiana tabacum). Biochem. Soc. Trans. 10: 353–354.Google Scholar
  8. Gianinazzi, S., 1978. Does the hypersensitive reaction in tobacco require the gene N? Abstr. Third Int. Congr. Plant Pathol., München, p. 222.Google Scholar
  9. Gianinazzi, S., 1982. Antiviral agents and inducers of virus resistance: analogies with interferon. In: R.K.S. Wood (Ed.), Active defense mechanisms in plants, Pp. 275–288. Plenum Press, New York.Google Scholar
  10. Gianinazzi, S., 1983. Genetic and molecular aspects of resistance induced by infections or chemicals. In: E.W. Nester & T. Kosuge (Eds), Plant-microbe interactions. Molecular and genetic perspectives, Vol. 1. Macmillan publishing Co., Inc., New York. (in press).Google Scholar
  11. Gianinazzi, S. & Schneider, C., 1979. Differential reactions to tobacco mosaic virus infection in ‘Samsun nn’ tobacco plants. II. A comparative ultrastructural study of the virus induced necroses with those of ‘Xanthi nc’. Phytopath. Z. 96: 313–323.Google Scholar
  12. Gianinazzi, S., Martin, C. & Vallée, J.C., 1970. Hypersensibilité aux virus, température et protéines solubles chez leNicotiana ‘Xanthi nc’. Apparition de nouvelles macromolécules lors de la répression de la synthèse virale. C.R. Acad. Sci. Paris D 270: 2383–2386.Google Scholar
  13. Gianinazzi, S., Deshayes, A., Martin, C. & Vernoy, R., 1977. Differential reaction to tobacco mosaic virus infection in ‘Samsun nn’ tobacco plants. I. Necrosis, mosaic symptoms and symptomless leaves following the ontogenic gradient. Phytopath. Z. 38: 347–354.Google Scholar
  14. Gianinazzi, S., Ahl, P., Cornu, A., Scalla, R. & Cassini, A., 1980. First report of host b-protein appearance in response to a fungal infection in tobacco. Physiol. Pl. Path. 16: 337–342.Google Scholar
  15. Jockusch, H., 1966. The rôle of host genes, temperature and polyphenoloxidase in the necrotization of TMV-infected tobacco tissue. Phytopath. Z. 55: 185–192.Google Scholar
  16. Loon, L.C. van & Kammen, A. van, 1970. Polyacrylamide disc electrophoresis of the soluble leaf proteins fromNicotiana tabacum var. ‘Samsun’ and ‘Samsun NN’. II. Changes in protein constitution after infection with tobacco mosaic virus. Virology 40: 199–211.CrossRefGoogle Scholar
  17. Martin, C., 1966. Contribution à l'étude du phénomène d'hypersensibilité au virus de la mosaïque du tabac. Bull. Soc. Franç. Physiol. Végét. 12: 345–354.Google Scholar
  18. Ross, A.F., 1961. Systemic acquired resistance induced by localized virus infections in plants. Virology 14: 340–358.CrossRefPubMedGoogle Scholar
  19. White, R.F., 1979. Acetylsalicylic acid (aspirin) induces resistance to tobacco mosaic virus in tobacco. Virology 99: 410–412.CrossRefPubMedGoogle Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1983

Authors and Affiliations

  • S. Gianinazzi
    • 1
  • Patricia Ahl
    • 1
  1. 1.Station d'Amélioration des PlantesInstitut National de la Recherche AgronomiqueDijon-CedexFrance

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