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Mass spectrometric techniques as aids in the diagnosis of gangrene in potatoes caused by Phoma exigua var. foveata

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Abstract

Current diagnosis of gangrene in potato tubers, caused byPhoma exigua var.foveata, is based on isolation and identification of the causal organism or on thin-layer chromatographic (TLC) detection of the anthraquinone pigment pachybasin (1-hydroxy-3-methylanthraquinone). In this paper alternative diagnostic approaches are described based on detection of specific ions by pyrolysis mass spectrometry (Py-MS), direct probe mass spectrometry (DIP-MS) and gas chromatography-mass spectrometry (GC-MS). The advantages and disadvantages of these methods are discussed in comparison with the TLC approach. GC-MS is a promising technique because of its high specificity, sensitivity and suitability for automation. Moreover, the MS approaches may lead to a reduction of total expertise time.

Samenvatting

De huidige diagnostiek van gangreen in aardappelknollen, veroorzaakt doorPhoma exigua var.foveata, is gebasserd op de isolatie en identifikatie van de schimmel of op de dunnelaag-chromatografische (TLC) bepaling van het pigment pachybasine (1-hydroxy-3-methylanthrachinon).

In dit artikel worden nieuwe diagnostische benaderingen beschreven die gebaseerd zijn op de detectie van specifieke ionen door middel van pyrolyse-massaspectrometrie (Py-MS), direct-probe-massaspectrometrie (DIP-MS) en gaschromatografie-massaspectrometrie (GC-MS). De voor- en nadelen van deze methoden worden behandeld en vergeleken met de dunnelaag-chromatografische methode.

GC-MS is een veelbelovende methode vanwege de grote specificiteit, gevoeligheid en geschiktheid voor automatisering. Op den duur kunnen de MS-methoden leiden tot een verkorting van de totale expertisetijd.

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Author notes

  1. W. Windig

    Present address: Biomaterials Profiling Center, University of Utah, P.O. Box 8089, 84108, Salt Lake City, Utah, USA

  2. J. Haverkamp

    Present address: Unilever Research Laboratory, P.O. Box 114, 3130 AC, Vlaardingen, the Netherlands

Authors and Affiliations

  1. Centraalbureau voor Schimmelcultures, P.O. Box 273, 3740 AG, Baarn, the Netherlands

    A. C. M. Weijman, G. W. van Eijk & H. J. Roeijmans

  2. FOM-Institute for Atomic and Molecular Physics, P.O. Box 4 1883, 1009 DB, Amsterdam, the Netherlands

    W. Windig & J. Haverkamp

  3. Research Institute for Plant Protection, P.O. Box 9060, 6700 GW, Wageningen, the Netherlands

    L. J. Turkensteen

Authors
  1. A. C. M. Weijman
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  2. G. W. van Eijk
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  3. H. J. Roeijmans
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  4. W. Windig
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  5. J. Haverkamp
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  6. L. J. Turkensteen
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Weijman, A.C.M., van Eijk, G.W., Roeijmans, H.J. et al. Mass spectrometric techniques as aids in the diagnosis of gangrene in potatoes caused by Phoma exigua var. foveata. Netherlands Journal of Plant Pathology 90, 107–115 (1984). https://doi.org/10.1007/BF01994516

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