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, Volume 34, Issue 1–2, pp 274–277 | Cite as

Structural and functional organization of the human neutrophil 60 kDa bactericidal/permeability-increasing protein

  • C. E. Ooi
  • J. Weiss
  • P. Elsbach
C. Gordon van Arman Scholarship Competition (Finalists)

Abstract

We have isolated, after limited proteolysis of the bactericidal/permeability-increasing protein (BPI) of human polymorphonuclear leukocytes (PMN), two fragments representing roughly the two halves of the BPI molecule. The 25 kDa N-terminal fragment possesses all the antibacterial activities of the 60 kDa parent protein, while the ca. 30 kDa C-terminal fragment has no detectable activity. The 25 kDa fragment is as potent on a molar basis as holo-human BPI against roughEscherichia coli, is more potent than holo-BPI against more resistant smoothE. coli, and retains the specificity of BPI toward Gram-negative bacteria. The findings suggest that all of the molecular determinants of the antibacterial properties of BPI reside within the N-terminal half of the molecule, implying a novel structural/functional organization for a cytotoxic protein.

Keywords

Antibacterial Activity Human Neutrophil Antibacterial Property Polymorphonuclear Leukocyte Functional Organization 

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Copyright information

© Birkhäuser Verlag 1991

Authors and Affiliations

  • C. E. Ooi
    • 1
  • J. Weiss
    • 1
  • P. Elsbach
    • 1
  1. 1.Departments of Medicine and MicrobiologyNew York University School of MedicineNew York

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