Skip to main content
SpringerLink
Log in

Eicosanoid production and phospholipase A2 secretion by peritoneal macrophages from rats with adjuvant-induced arthritis

  • PLA2/5-Lipoxygenase
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

The effect of adjuvant-induced arthritis on rat peritoneal macrophage (RPM) function with respect to [14C]arachidonic acid (AA) release, leukotriene B4 (LTB4) and prostaglandin E2 (PGE2) production, and secreted phospholipase A2 (PLA2) activity was investigated. Twice as many cells were lavaged from the peritoneal cavity of arthritic rats 21 days post-adjuvant injection than were lavaged from normal rats. PGE2 production was increased two-fold in Ca++ ionophore-stimulated RPM from the adjuvant animals as compared with RPM from control animals. However, PLA2 secretion, LTB4 production and [14C]AA release were unchanged. These results suggest that PGE2 production, rather than LTB4 production or PLA2 secretion, is preferentially enhanced in Ca++ ionophore-stimulated RPM from arthritic rats and may, therefore, reflect a major role for PGE2 in adjuvant-induced arthritis. However, the presence of increase numbers of macrophages and their associated products, including PLA2 and LTB4, may also contribute to the inflammatory process in this disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. P. Vadas, W. Pruzanski,Biology of Disease-Role of Secretory Phospholipases A2 in the Pathobiology of Disease. Lab. Invest.55, 391–404 (1986).

    PubMed  Google Scholar 

  2. M. Waite,The Phospholipases. InHandbook of Lipid Research (Ed. D. J. Hanahan) Plenum Press, NY (1987).

    Google Scholar 

  3. I. G. Otterness, M. L. Bliven,Laboratory Models for Testing Nonsteroidal Antiinflammatory Drugs. InNonsteroidal Antiinflammatory Drugs. (Ed. J. G. Lombardino) pp. 111–252, John Wiley u. Sons, NY (1985).

    Google Scholar 

  4. C. M. Pearson, F. D. Wood,Studies of arthritis and other lesions induced in rats by the injection of mycobacterial adjuvant. VIII. Pathologic details of the arthritis and spondylitis. Am. J. Pathol.42, 73–86 (1963).

    PubMed  Google Scholar 

  5. K. A. Hill, S. Whately,A Simple Rapid Method for DNA Quantitation. FEBS Letters56, 20–23 (1975).

    Article  PubMed  Google Scholar 

  6. P. Vadas, E. Stefanski, W. Pruzanski,Influence of plasma protein on activity of proinflammatory enzyme phospholipase A 2. Inflammation10, 183–193 (1986).

    Article  PubMed  Google Scholar 

  7. W. J. Johnson, K. A. Muirhead, P. C. Meunier, B. J. Votta, T. C. Schmitt, M. J. DiMartino, N. Hanna,Macrophage Activation in Rat Models of Inflammation and Arthritis. Systemic Activation Precedes Arthritis Induction and Progression. Arthritis and Rheumatism29, 1122–1130 (1986).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology, Hoffmann-La Roche Inc., 07110-1199, Nutley, NJ, USA

    C. E. T. Bak, C. M. Anderson, A. C. Hanglow & D. W. Morgan

Authors
  1. C. E. T. Bak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. M. Anderson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. C. Hanglow
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. W. Morgan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bak, C.E.T., Anderson, C.M., Hanglow, A.C. et al. Eicosanoid production and phospholipase A2 secretion by peritoneal macrophages from rats with adjuvant-induced arthritis. Agents and Actions 34, 81–83 (1991). https://doi.org/10.1007/BF01993244

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01993244

Keywords

Search

Navigation