Abstract
After 72 hrs in culture, unseparated spleen cells from rats with adjuvant-induced arthritis (AA) stimulated with high concentrations of Con A (>0.63 μg/ml) leaked more lactate dehydrogenase (LDH) than did either unstimulated cultures or cultures stimulated with lower concentrations of Con A. This reflects and increase in dead or dying cells at concentrations of Con A >0.63 μg/ml. Since Con A did not increase LDHlleakage in macrophage-depleted cultures of AA splenocytes, the decreased viability in unseparated, Con A-stimulated cultures appears to be a macrophage-dependent phenomenon. Since the increase in LDH release at high concentrations of Con A paralleled the decrease in [3H]-thymidine incorporation, these results suggest that Con A (>0.63 μg/ml) induces macrophages from AA rats to kill splenic lymphocytes in culture.
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References
A. C. Hanglow, H. Bachmann, M. Rosenberger and J. W. Coffey,Effects of a novel anti-inflammatory retinoid-like 2,4,6,8-nonatetraenoic acid on the immunological changes associated with adjuvant-induced arthritis. Int. J. Immunopharmac.12, 703–712 (1990).
L. Binderup, E. Bramn and E. Arrigoni-Martelli,Splenic suppressor cells in adjuvant arthritis; effect of d-penicillamine. Agents and Actions7, 199–203 (1980).
H. U. Bergmeyer and E. Bernt,Colorimetric assay with l-lactate, NAD, phenazine methosulphate and INT. InMethods of enzymatic analysis. Vol. 2, pp. 579–582, Verlag Chemie International, Florida 1974.
B. Dewald and M. Baggiolini,Methods for assessing exocytosis by neutrophil leukocytes. Methods in Enzymology132, 274–277 (1986).
L. Binderup,Lymphocyte-macrophage co-operation during induction of T-suppressor cell activity in rats with adjuvant arthritis. Ann. Rheum. Dis.42, 687–692 (1983).
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Hanglow, A.C., Bachmann, H. & Coffey, J.W. Macrophage-mediated killing of splenic lymphocytes in adjuvant-induced arthritis. Agents and Actions 34, 11–15 (1991). https://doi.org/10.1007/BF01993224
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DOI: https://doi.org/10.1007/BF01993224