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, Volume 33, Issue 1–2, pp 185–188 | Cite as

Mast cell regulatory effect on lymphoid cell proliferation

  • I. S. Gushchin
  • N. S. Prozorovsky
  • O. A. Yamshchikova
  • A. V. Kiselev
Histamine in the Modulation of Cell Function and Cancer

Abstract

It has been shown rat mast cells (MC) can modulate lymphocyte proliferationin vitro. Depending on concentrations tested both serosal MC and their supernatants enhanced the spontaneous and T-mitogen-induced proliferation of spleen and lymph node cells. In addition T-mitogen-induced thymocyte proliferation was also increased. The enhancing effect of MC on lymphoid cell proliferation appeared after MC and lymphocytes were cocultured for 24, 48 or 72 h. The highest enhancing action of MC was observed when MC and lymphocytes were plated simultaneously. In contrast, when MC were added 24 or 48 h after the start of lymphocyte culture, the enhancing action of MC decreased or was abolished, respectively. No dependence was found between histamine concentration in MC supernatants and the enhancing activity of supernatants. After chromatographic separation of MC supernatants the fractions with molecular weights between 1–6 KDa augmented lymphoid cell proliferation.

Keywords

Lymph Node Molecular Weight Cell Proliferation Mast Cell Histamine 

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References

  1. [1]
    S. Ahlstedt, E. Olaisson, J. Thellin and B. Bjorkesten,Appearance of mast cells in bone marrow, peripheral blood and spleen of immunized rats. Int. Arch. Allergy Appl. Immunol.80, 122–126 (1986).PubMedGoogle Scholar
  2. [2]
    S. K. Kops, R. E. Ratzlaff, R. Meade, G. M. Iverson and P. W. Askenase,Interaction of antigen-specific T cell factors with unique “receptors” on surface of mast cells: demonstration in vitro by an indirect rosetting technique. J. Immunol.136, 4514–4524 (1986).Google Scholar
  3. [3]
    A. Ferguson, A. G. Cummins, G. H. Munro, S. Gibson and H. R. P. Miller,Roles of mucosal mast cells in cell-mediated immunity. Ann. Allergy59, 40–43 (1987).PubMedGoogle Scholar
  4. [4]
    M. M. Khan, S. Strober and K. L. Melmon,Regulatory effects of mast cells on lymphoid cells: the role of histamine type 1 receptors in the interaction between mast cells, helper T cells and natural suppressor cells. Cell. Immunol.103, 41–53 (1986).PubMedGoogle Scholar
  5. [5]
    H. A. Fallah, J. L. Maillard and G. A. Voisin,Regulatory mast cells. 1. Suppressive action of their products on an invitro primary immune reaction. Ann. Immunol.126C, 669–682 (1975).Google Scholar
  6. [6]
    B. B. Fredholm, I. S. Gushchin, K. Elvin, G. Schwab and B. Uvnäs,Cyclic AMP independent inhibition by papaverine of histamine release induced by compound 48/80. Biochem. Pharmacol.25, 1583–1588 (1976).PubMedGoogle Scholar
  7. [7]
    S. J. Galli and A. M. Dvorak,What do mast cells have to do with delayed hypersensitivity? Lab. Invest.50, 365–370 (1984).PubMedGoogle Scholar
  8. [8]
    I. S. Nikolsky, D. F. Gluzman and F. B. Filchakov,Cytochemical peculiarities of cells forming mastolymphocytic rosettes. Cytology (USSR)27, 1277–1279 (1985).Google Scholar
  9. [9]
    M. Plaut, J. H. Pierce, C. J. Watson, J. Hanley-Hyde, R. P. Nordan and W. E. Paul,Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon R1 or to calcium ionophores. Nature339, 64–67 (1989).PubMedGoogle Scholar
  10. [10]
    M. Steffen, M. Abboud, G. K. Potter, Y. P. Yung and M. A. S. Moore,Presence of tumour necrosis factor or related factor in human basophil/mast cells. Immunol.66, 445–450 (1989).Google Scholar

Copyright information

© Birkhäuser Verlag 1991

Authors and Affiliations

  • I. S. Gushchin
    • 1
  • N. S. Prozorovsky
    • 1
  • O. A. Yamshchikova
    • 1
  • A. V. Kiselev
    • 1
  1. 1.Institute of ImmunologyMoscowUSSR

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