Mast cell regulatory effect on lymphoid cell proliferation
- 15 Downloads
It has been shown rat mast cells (MC) can modulate lymphocyte proliferationin vitro. Depending on concentrations tested both serosal MC and their supernatants enhanced the spontaneous and T-mitogen-induced proliferation of spleen and lymph node cells. In addition T-mitogen-induced thymocyte proliferation was also increased. The enhancing effect of MC on lymphoid cell proliferation appeared after MC and lymphocytes were cocultured for 24, 48 or 72 h. The highest enhancing action of MC was observed when MC and lymphocytes were plated simultaneously. In contrast, when MC were added 24 or 48 h after the start of lymphocyte culture, the enhancing action of MC decreased or was abolished, respectively. No dependence was found between histamine concentration in MC supernatants and the enhancing activity of supernatants. After chromatographic separation of MC supernatants the fractions with molecular weights between 1–6 KDa augmented lymphoid cell proliferation.
KeywordsLymph Node Molecular Weight Cell Proliferation Mast Cell Histamine
Unable to display preview. Download preview PDF.
- S. K. Kops, R. E. Ratzlaff, R. Meade, G. M. Iverson and P. W. Askenase,Interaction of antigen-specific T cell factors with unique “receptors” on surface of mast cells: demonstration in vitro by an indirect rosetting technique. J. Immunol.136, 4514–4524 (1986).Google Scholar
- H. A. Fallah, J. L. Maillard and G. A. Voisin,Regulatory mast cells. 1. Suppressive action of their products on an invitro primary immune reaction. Ann. Immunol.126C, 669–682 (1975).Google Scholar
- I. S. Nikolsky, D. F. Gluzman and F. B. Filchakov,Cytochemical peculiarities of cells forming mastolymphocytic rosettes. Cytology (USSR)27, 1277–1279 (1985).Google Scholar
- M. Steffen, M. Abboud, G. K. Potter, Y. P. Yung and M. A. S. Moore,Presence of tumour necrosis factor or related factor in human basophil/mast cells. Immunol.66, 445–450 (1989).Google Scholar