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, Volume 33, Issue 1–2, pp 84–87 | Cite as

Molecular basis for cellular effects of naturally occurring polyamines

  • J. -L. Bueb
  • M. Mousli
  • Y. Landry
Histamine Release from Mast Cells and Basophils

Abstract

The naturally occurring polyamines, putrescine, spermidine and spermine, and the analogue cadaverine, induce a dose-dependent histamine release from rat peritoneal mast cells. Spermine was the most active among these polycationic metabolites, followed by spermidine and putrescine. The histamine release was inhibited by a 2 h pretreatment of the cells with pertussis toxin (100 ng/ml), demonstrating the involvement of a pertussis toxin-sensitive GTP-binding regulatory protein during the exocytotic process. Experiments performed with purified Go/Gi proteins reconstituted into phospholipid vesicles showed a direct stimulation of GTPase activity by the polyamines. This direct stimulation of G proteins and the consequent activation of the coupled effectors may represent a new mechanism of action for natural polyamines controlling receptor-dependent processes.

Keywords

Mast Cell Histamine Pertussis Spermine Putrescine 

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Copyright information

© Birkhäuser Verlag 1991

Authors and Affiliations

  • J. -L. Bueb
    • 1
  • M. Mousli
    • 1
  • Y. Landry
    • 1
  1. 1.Laboratoire de NeuroimmunopharmacologieUniversité Louis Pasteur — Strasbourg I, Faculté de PharmacieIllkirch CedexFrance

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