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, Volume 33, Issue 1–2, pp 84–87 | Cite as

Molecular basis for cellular effects of naturally occurring polyamines

  • J. -L. Bueb
  • M. Mousli
  • Y. Landry
Histamine Release from Mast Cells and Basophils

Abstract

The naturally occurring polyamines, putrescine, spermidine and spermine, and the analogue cadaverine, induce a dose-dependent histamine release from rat peritoneal mast cells. Spermine was the most active among these polycationic metabolites, followed by spermidine and putrescine. The histamine release was inhibited by a 2 h pretreatment of the cells with pertussis toxin (100 ng/ml), demonstrating the involvement of a pertussis toxin-sensitive GTP-binding regulatory protein during the exocytotic process. Experiments performed with purified Go/Gi proteins reconstituted into phospholipid vesicles showed a direct stimulation of GTPase activity by the polyamines. This direct stimulation of G proteins and the consequent activation of the coupled effectors may represent a new mechanism of action for natural polyamines controlling receptor-dependent processes.

Keywords

Mast Cell Histamine Pertussis Spermine Putrescine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1991

Authors and Affiliations

  • J. -L. Bueb
    • 1
  • M. Mousli
    • 1
  • Y. Landry
    • 1
  1. 1.Laboratoire de NeuroimmunopharmacologieUniversité Louis Pasteur — Strasbourg I, Faculté de PharmacieIllkirch CedexFrance

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