Abstract
Several vanadium compounds were tested for their ability to induce in vitro hemolysis of vitamin E-deficient hamster erythrocytes. Free vanadyl caused hemolysis in Hepes buffer but not in Tris or phosphate buffer, while hemolysis was inhibited by catalase, chelators such as deferoxamine mesylate and EDTA, and hydroxyl radical scavengers such as ethanol andd-mannitol. Although metavanadate itself could not induce hemolysis, metavanadate with NAD(P)H caused hemolysis in Hepes buffer only, and superoxide dismutase prevented it. Hydrogen peroxide, hydroxyl radical and Hepes radical were involved in vanadyl-induced hemolysis; superoxide anion was further involved in metavanadate plus NAD(P)H-induced hemolysis. Vitamin E prevented hemolysis under both conditions.
Similar content being viewed by others
References
French, R. J., and Jones, P. J. H., Life Sci.52 (1993) 341.
Lagerkvist, B., Nordberg, G. F., and Vouk, V., in: Handbook on the Toxicology of Metals, vol. 2, p. 638. Eds L. Friberg, G. F. Nordberg and V. B. Vouk. Elsevier, Amsterdam-New York-Oxford 1986.
Byerrum, R. U., in: Metals and Their Compounds in the Environment, p. 1289. Ed. E. Merian, VCH Verlag, Weinheim-New York-Basel-Cambridge 1991.
Rehder, D., BioMetals5 (1992) 3.
Willsky, G. R., in: Vanadium in Biological Systems, p. 1. Ed. N. D. Chasteen, Kluwer, Dordrecht-Boston-London 1990.
Breit, G. N., and Wanty, R. B., Chem. Geol.91 (1991) 83.
Nechay, B. R., Nanninga, L. B., Nechay, P. S. E., Post, R. L., Grantham J., Macara, I. G., Kubena, L. F., Phillips, T. D., and Nielsen, F. H., Fedn Proc.45 (1986) 123.
Hamada, T., Proc. Symp. Trace Nutrients Research (Kyoto)8 (1991) 125.
Hansard, II, S. L., Ammerman, C. B., and Henry, P. R., J. Anim, Sci.55 (1982) 350
Hathcock, J. N., Hill, C. H., and Matrone, G., J. Nutr.82, (1964) 106.
Nielsen, F. H., in: Trace Elements in Human and Animal Nutrition, vol. 1, p. 275. Ed. W. Mertz, Academic Press, San Diego-London-Sydney-Tokyo 1986.
Phillips, T. D., Nechay, B. R., and Heidelbaugh, N. D., Fedn Proc.42 (1983) 2969.
Hogan, G. R., Experientia46 (1990) 444.
Keller, R. J., Sharma, R. P., Grover, T. A., and Piette, L. H., Arch. Biochem. Biophys.265 (1988) 524.
Ramasarma, T., MacKellar, W. C., and Crane, F. L., Biochim. biophys. Acta646 (1981) 88.
Hamada, T., and Matsumoto, M., Experientia 36 (1980) 978.
Hamada, T., Furuya, M., and Hodate, K., Experientia38 (1982) 462.
Hamada, T., Hodate, K., and Nakayama, E., Experientia40 (1984) 258.
Hamada, T., and Nakayama, E., Proc. Symp. Trace Nutrients Research (Kyoto) 9 (1992) 177.
Byczkowski, J. Z., and Kulkarni, A. P., Biochim. Biophys. Acta 1125 (1992) 134.
Fuhrmann, G. F., Hüttermann, J., and Knauf, P. A., Biochim. biophys. Acta 769 (1984) 130.
Liochev, S., and Ivancheva, E., Free Rad. Res. Comms. 14 (1991) 335.
Keller, R. J., Coulombe, Jr., R. A., Sharma, R. P., Grover, T. A., and Piette, L. H., Free Rad. Biol. Med. 6 (1989) 15.
Diplock, A. T., Am. J. Clin. Nutr. 53 (1991) 189S.
Grady, J. K., Chasteen, N. D., and Harris, D. C., Analyt. Biochem. 173 (1988) 111.
Liochev, S. I., and Fridovich, I., Arch Biochem. Biophys.291 (1991) 379.
Simpson, J. A., Cheeseman, K. H., Smith, S. E., and Dean, R. T., Biochem. J.254 (1988) 519.
Darr, D., and Fridovich, I., Archs Biochem. Biophys.243 (1985) 220.
Liochev, S. I., and Ivancheva, E. A., Free Rad. biol. Med.5 (1988) 349.
Zaporowska, H., and Wasilewski, W., Comp. Biochem. Physiol.93C (1989) 175.
Keller, R. J., Rush, J. D., and Grover, T. A., J. inorg. Biochem.41 (1991) 269.
Domingo, J. L., Gomez, M., Llobet, J. M., and Corbella, J., Toxicology62 (1990) 203.
Hamada, T., Analyt. Biochem.43 (1991) 490.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hamada, T. Vanadium induced hemolysis of vitamin E deficient erythrocytes in Hepes buffer. Experientia 50 (Suppl 1), 49–53 (1994). https://doi.org/10.1007/BF01992049
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01992049