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, Volume 36, Issue 1–2, pp 66–69 | Cite as

The pneumotoxicant paraquat potentiates IL-1 and TNF production by human mononuclear cells

  • A. Erroi
  • M. Bianchi
  • P. Ghezzi
Inflammation and Immunomodulation

Abstract

Interleukin 1 (IL-1) and tumor necrosis factor (TNF) have been implicated in the pathogenesis of bleomycin- and silica-induced lung damage. We have studied the effect of paraquat (PQ), a well-known pneumotoxicant, on IL-1 and TNF production by human peripheral blood mononuclear cells from different healthy donors stimulated with endotoxin. PQ (100 μM) potentiated IL-1 production (2–40 fold) and TNF production (2–18 fold). It is, therefore, possible that IL-1 and TNF are also involved in the pneumotoxic action of PQ.

Keywords

Tumor Necrosis Tumor Necrosis Factor Mononuclear Cell Peripheral Blood Mononuclear Cell Healthy Donor 

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References

  1. [1]
    R. D. Kimbrough and T. B. Gaines,Toxicity of paraquat to rats and its effect on rat lungs. Tox. App. Pharmacol.17, 679–690 (1970).Google Scholar
  2. [2]
    P. Smith, D. Heath and J. M. Kay,The pathogenesis and structure of paraquat-induced pulmonary fibrosis in rats. J. Pathol.114, 57–67 (1974).PubMedGoogle Scholar
  3. [3]
    G. M. Copland, A. Kolin and H. S. Shulman,Fatal pulmonary intra-alveolar fibrosis after paraquat ingestion. New Engl. J. Med.291, 290–292 (1974).PubMedGoogle Scholar
  4. [4]
    P. F. Piguet, M. A. Collart, G. E. Grau, Y. Kapanci and P. Vassalli,Tumor necrosis factor/cachectin plays a key role in bleomycin-induced pneumopathy and fibrosis. J. Exp. Med.170, 655–663 (1989).PubMedGoogle Scholar
  5. [5]
    P. F. Piguet, M. A. Collart, G. E. Grau, A.-P. Sappino and P. Vassalli,Requirement of tumor necrosis factor for development of silica-induced pulmonary fibrosis. Nature344, 245–247 (1990).PubMedGoogle Scholar
  6. [6]
    C. A. Dinarello,Biology of interleukin 1. FASEB J.2, 108–115 (1988).PubMedGoogle Scholar
  7. [7]
    B. Beutler and A. Cerami,Cachectin: More than a tumor necrosis factor. New Engl. J. Med.316, 379–385 (1987).PubMedGoogle Scholar
  8. [8]
    B. J. Sugarman, B. B. Aggarwal, P. E. Hass, I. S. Figari, M. A. Palladino Jr. and H. M. Shepard,Recombinant human tumor necrosis factor-α: Effects on proliferation of normal and transformed cells in vitro. Science230, 943–945 (1985).PubMedGoogle Scholar
  9. [9]
    J. A. Schmidt, S. B. Mizel, D. Cohen and I. Green,Interleukin 1, a potential regulator of fibroblast proliferation. J. Immunol.128, 2177–2182 (1982).PubMedGoogle Scholar
  10. [10]
    J. A. Schmidt, C. N. Oliver, J. L. Lepe-Zuniga, I. Green and I. Gery,Silica-stimulated monocytes release fibroblast proliferation factors identical to interleukin 1. A potential role for interleukin 1 in the pathogenesis of silicosis. J. Clin. Invest.73, 1462–1472 (1984).PubMedGoogle Scholar
  11. [11]
    K. E. Stephens, A. Ishizaka, J. W. Larrick and T. A. Raffin,Tumor necrosis factor causes increased pulmonary perme-ability and edema. Comparison to septic acute lung injury. Am. Rev. Respir. Dis.137, 1364–1370 (1988).PubMedGoogle Scholar
  12. [12]
    A. Suwabe, K. Takahashi, S. Yasui, S. Arai and F. Sendo,Bleomycin-stimulated hamster alveolar macrophages release interleukin-1. Am. J. Pathol.132, 512–520 (1988).PubMedGoogle Scholar
  13. [13]
    P. J. Lisi, C. W. Chu, G. A. Koch, S. Endres, G. Lonnemann and C. A. Dinarello,Development and use of a radioimmunoassay for human interleukin-1 beta. Lymphokine Res.6, 229–244 (1987).PubMedGoogle Scholar
  14. [14]
    B. B. Aggarwal, W. J. Kohr, P. E. Hass, B. Moffat, S. A. Spencer, W. J. Henzel, T. S. Bringman, G. E. Nedwin, D. V. Goeddel and R. N. Harkins,Human tumor necrosis factor. Production, purification, and characterization. J. Biol. Chem.260, 2345–2354 (1985).PubMedGoogle Scholar
  15. [15]
    G. L. Plaa and H. Witschi,Chemicals, drugs, and lipid peroxidation. Ann. Rev. Pharmacol. Toxicol.16, 125–141 (1976).Google Scholar
  16. [16]
    G. Chaudhri and I. A. Clark,Reactive oxygen species facilitate the in vitro and in vivo lipopolysaccharide-induced release of tumor necrosis factor. J. Immunol.143, 1290–1294 (1989).PubMedGoogle Scholar
  17. [17]
    L. M. Colletti, D. G. Remick, G. D. Burtch, S. L. Kunkel, R. M. Strieter and D. A. Campbell Jr.,Role of tumor necrosis factor-α in the pathophysiologic alterations after hepatic ischemia/reperfusion injury in the rat. J. Clin. Invest.85, 1936–1943 (1990).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1992

Authors and Affiliations

  • A. Erroi
    • 1
  • M. Bianchi
    • 1
  • P. Ghezzi
    • 1
  1. 1.Istituto di Ricerche Farmacologiche “Mario Negri”MilanItaly

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