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Activation of neutrophil migration by dioctanoyl-sn-glycerol and fMet-Leu-Phe is controlled by different pathways

  • General Inflammation
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Abstract

Migration activated by fMet-Leu-Phe is inhibited by GTP[S] and is little affected by protein kinase C inhibitors. We investigated the effects of GTP[S] and the protein kinase C inhibitor AMG-C16 on dioctanoyl-sn-glycerol (DiC8)-activated migration of rabbit neutrophils and compared them with the effects on fMet-Leu-Phe-activated migration and random migration. GTP[S] did not inhibit DiC8-activated migration or random migration but inhibited fMet-Leu-Phe-activated migration. AMG-C16 gave a strong inhibition of DiC8-activated migration but had only a small effect on fMet-Leu-Phe-activated migration and random migration. When fMet-Leu-Phe and DiC8 were added together in suboptimal concentrations an additive effect was found. Pretreatment with the diacylglycerol kinase inhibitor R59022 enhanced random migration. The enhancement was completely inhibited by AMG-C16 and was unaffected by GTP[S]. These findings suggest that DiC8-activated migration and fMet-Leu-Phe-activated migration are controlled by different pathways.

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Abbreviations

GTP[S]:

guanosine-5′-O-[3-thio]triphosphate

fMet-Leu-Phe:

formylmethionyl-leucylphenylalanine

AMG-C16 :

1-O-hexadecyl-2-methyl-sn-glycerol

DiC8:

dioctanoyl-sn-glycerol

R59022:

6-(2-(4-((p-fluorophenyl)-phenylmethylene)-1-piperidinyl)-ethyl)-7-methyl-5-H-thiazolo-(3,2-a)-pyrimidine-5-one

References

  1. G. J. J. C. Boonen, J. VanSteveninck, B. M. de Koster and J. G. R. Elferink,Inhibition of neutrophil migration by guanine nucleotides. Agents and Actions32, 100–102 (1991).

    PubMed  Google Scholar 

  2. J. A. Badwey, J. M. Robinson, P. G. Heyworth and J. T. Curnutte,1,2-Dioctanoyl-sn-glycerol can stimulate neutrophils by different mechanisms. J. Biol. Chem.264, 20676–20682 (1989).

    PubMed  Google Scholar 

  3. J.-L. Mege, W. Tao, T. F. P. Molski, J. Gomez-Cambronero, C.-K. Huang, E. L. Becker and R. I. Sha'afi,Diacylglycerol kinase inhibitor R59022 and stimulated neutrophil responses. Am. J. Physiol.255, C589-C594 (1988).

    PubMed  Google Scholar 

  4. S. H. Zigmond and J. G. Hirsch,Leukocyte locomotion and chemotaxis: New methods for evaluation and demonstration of a cell-derived chemotactic factor. J. Exp. Med.137, 387–410 (1973).

    Google Scholar 

  5. IJ. M. Kramer, R. L. van der Bend, A. T. J. Tool, W. J. Blitterswijk, D. Roos and A. J. Verhoeven,1-O-hexadecyl-2-methylglycerol, a novel inhibitor of protein kinase C, inhibits the respiratory burst in human neutrophils. J. Biol. Chem.264, 5876–5884.

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Authors and Affiliations

  1. Department of Medical Biochemistry, University of Leiden, POB 9503, 2300 RA, Leiden, The Netherlands

    G. J. J. C. Boonen, B. M. de Koster & J. G. R. Elferink

Authors
  1. G. J. J. C. Boonen
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  2. B. M. de Koster
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  3. J. G. R. Elferink
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Boonen, G.J.J.C., de Koster, B.M. & Elferink, J.G.R. Activation of neutrophil migration by dioctanoyl-sn-glycerol and fMet-Leu-Phe is controlled by different pathways. Agents and Actions 38 (Suppl 2), C130–C132 (1993). https://doi.org/10.1007/BF01991161

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  • DOI: https://doi.org/10.1007/BF01991161

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